ArticleScientific reports2017
Preferential suppression of Anopheles gambiae host sequences allows detection of the mosquito eukaryotic microbiome.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Enhanced blood parasite species identification using V4-V9 18S rDNA barcoding by universal primers on a nanopore platform.Scientific reports · 2025Article
- CRISPR-Cas9-mediated host signal reduction for 18S metabarcoding of host-associated eukaryotes.Molecular ecology resources · 2024Article
- Interkingdom interactions shape the fungal microbiome of mosquitoes.Animal microbiome · 2024Article
- Improved 18S rDNA profiling of parasite communities in salmonid tissues using a host blocking primer.Parasitology research · 2024Article
- VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages.Nature communications · 2024Article
- Primer pairs, PCR conditions, and peptide nucleic acid clamps affect fungal diversity assessment from plant root tissues.Mycology · 2024Article
- Microbiota is structured by gut regions, life stage, and diet in the Black Soldier Fly (Frontiers in microbiology · 2023Article
- Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS).Microbiome · 2021Article
- Applications of Blocker Nucleic Acids and Non-Metazoan PCR Improves the Discovery of the Eukaryotic Microbiome in Ticks.Microorganisms · 2021Article
- Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels.Pathogens (Basel, Switzerland) · 2021Article
- A Sarcina bacterium linked to lethal disease in sanctuary chimpanzees in Sierra Leone.Nature communications · 2021Article
- Considerations for mosquito microbiome research from the Mosquito Microbiome Consortium.Microbiome · 2021Review
- Comprehensive Ecological and Geographic Characterization of Eukaryotic and Prokaryotic Microbiomes in AfricanFrontiers in microbiology · 2021Article
- The Axenic and Gnotobiotic Mosquito: Emerging Models for Microbiome Host Interactions.Frontiers in microbiology · 2021Review
- Mosquito Mycobiota: An Overview of Non-Entomopathogenic Fungal Interactions.Pathogens (Basel, Switzerland) · 2020Review
- Exposure of Anopheles mosquitoes to trypanosomes reduces reproductive fitness and enhances susceptibility to Plasmodium.PLoS neglected tropical diseases · 2020Article
- Use of Microbiota to Fight Mosquito-Borne Disease.Frontiers in genetics · 2020Review
- Improved 18S and 28S rDNA primer sets for NGS-based parasite detection.Scientific reports · 2019Article
- Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR).Biology methods & protocols · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Anopheles mosquitoes are vectors of the human malaria parasite, Plasmodium falciparum. The vector microbiota is a likely factor influencing parasite transmission. The prokaryotic microbiota of mosquitoes is efficiently surveyed by sequencing of hypervariable regions of the 16s ribosomal RNA (rRNA) gene. However, identification of the eukaryotic microbiota by targeting the 18s rRNA gene is challenging due to simultaneous amplification of the abundant 18s rRNA gene target in the mosquito host. Consequently, the eukaryotic microbial diversity of mosquitoes is vastly underexplored. An efficient methodology is needed to identify this component of the microbiota, expected to include relatives of Plasmodium. Here, we use defined panels of Anopheles samples from West Africa to test two experimental PCR clamp approaches to maximize the specific amplification of 18s rRNA gene hypervariable regions from eukaryotic microbes: anneal-inhibiting blocking primers and peptide-nucleic acid (PNA) oligonucleotide blockers. Of the two, PNA blockers were the only efficient blocking strategy, allowing a reduction of mosquito 18s rRNA gene sequences by more than 80% for the V4 hypervariable region. These PNA blockers will facilitate taxonomic profiling of the eukaryotic microbiota of the A. gambiae species complex, and contribute to a better understanding of microbial influence upon immunity and pathogen infection.
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Registered trials
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