ArticlePlant biotechnology journal2018
A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 164 citations in OpenAlex.
- Synthetic Biology and Metabolic Engineering of Microalgae for Sustainable Lipid and Terpenoid Production: An Updated Perspective.Plant biotechnology journal · 2026Review
- A modular synthetic biology toolkit unlocks metabolic engineering of the industrially relevant alga Nannochloropsis.Advanced biotechnology · 2026Article
- Microalgal Synthetic Biotechnology: From Genetic Models to Deployable Photosynthetic Chassis.Research (Washington, D.C.) · 2026Review
- Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025Review
- Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system.Scientific reports · 2025Article
- Microbial Bioprocessing of Food and Agro-Industrial Residues for a Sustainable Circular Economy.Indian journal of microbiology · 2025Review
- Systematic approach for dissecting promoters and designing transform systems in microalgae.Microbial cell factories · 2025Article
- Last Decade Insights in Exploiting Marine Microorganisms as Sources of New Bioactive Natural Products.Marine drugs · 2025Review
- Marine Phytoplankton Bioactive Lipids and Their Perspectives in Clinical Inflammation.Marine drugs · 2025Review
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- Comparative Analysis of the Biochemical and Molecular Responses ofMarine drugs · 2024Article
- Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan.Nature communications · 2024Article
- Review
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- Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities.Biotechnology for biofuels and bioproducts · 2024Review
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Engineering the Metabolic Landscape of Microorganisms for Lignocellulosic Conversion.Microorganisms · 2023Review
- Zeaxanthin epoxidase is involved in the carotenoid biosynthesis and light-dependent growth of the marine alga Nannochloropsis oceanica.Biotechnology for biofuels and bioproducts · 2023Article
- Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica.Microbial cell factories · 2023Article
- CRISPR-based bioengineering in microalgae for production of industrially important biomolecules.Frontiers in bioengineering and biotechnology · 2023Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nannochloropsis oceanica is an oleaginous microalga rich in ω3 long-chain polyunsaturated fatty acids (LC-PUFAs) content, in the form of eicosapentaenoic acid (EPA). We identified the enzymes involved in LC-PUFA biosynthesis in N. oceanica CCMP1779 and generated multigene expression vectors aiming at increasing LC-PUFA content in vivo. We isolated the cDNAs encoding four fatty acid desaturases (FAD) and determined their function by heterologous expression in S. cerevisiae. To increase the expression of multiple fatty acid desaturases in N. oceanica CCMP1779, we developed a genetic engineering toolkit that includes an endogenous bidirectional promoter and optimized peptide bond skipping 2A peptides. The toolkit also includes multiple epitopes for tagged fusion protein production and two antibiotic resistance genes. We applied this toolkit, towards building a gene stacking system for N. oceanica that consists of two vector series, pNOC-OX and pNOC-stacked. These tools for genetic engineering were employed to test the effects of the overproduction of one, two or three desaturase-encoding cDNAs in N. oceanica CCMP1779 and prove the feasibility of gene stacking in this genetically tractable oleaginous microalga. All FAD overexpressing lines had considerable increases in the proportion of LC-PUFAs, with the overexpression of Δ12 and Δ5 FAD encoding sequences leading to an increase in the final ω3 product, EPA.
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