ArticleJournal of visualized experiments : JoVE2017
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
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- Optimizing multiomics sample preparation: comparative evaluation of extraction protocols for HepG2 cells.Analytical and bioanalytical chemistry · 2026Article
- Disruption of the karrikin receptor DWARF 14 LIKE (D14L) gene leads to distinct effects on root and shoot growth, and reprogramming of central metabolism in rice.Journal of experimental botany · 2025Article
- Review
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- Non-targeted metabolomics reveals fatty acid and associated pathways driving resistance to whitefly and tomato leafminer in wild tomato accessions.Scientific reports · 2025Article
- A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs.bioRxiv : the preprint server for biology · 2024Article
- Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization ofJournal of proteome research · 2024Article
- Biomolecular characterization of 3500-year-old ancient Egyptian mummification balms from the Valley of the Kings.Scientific reports · 2023Article
- Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-'omics analysis of the liver metabolic response to viral infection.RNA biology · 2023Article
- Heterosis for capsacinoids accumulation in chili pepper hybrids is dependent on parent-of-origin effect.Scientific reports · 2022Article
- Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation.The Plant cell · 2022Article
- Metabolomics insight into the influence of environmental factors in responses of freshwater biofilms to the model herbicide diuron.Environmental science and pollution research international · 2022Article
- 2',3'-cAMP treatment mimics the stress molecular response in Arabidopsis thaliana.Plant physiology · 2022Article
- Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants.Plant cell reports · 2022Review
- In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide.Journal of structural biology: X · 2022Article
- A Comparative Study of the Antihypertensive and Cardioprotective Potentials of Hot and Cold Aqueous Extracts ofFrontiers in pharmacology · 2022Article
- Application of a comprehensive metabolomics approach for the selection of flaxseed varieties with the highest nutritional and medicinal attributes.Journal of food and drug analysis · 2021Article
- Three-in-One Simultaneous Extraction of Proteins, Metabolites and Lipids for Multi-Omics.Frontiers in genetics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding of complex biological systems requires the measurement, analysis and integration of multiple compound classes of the living cell, usually determined by transcriptomic, proteomic, metabolomics and lipidomic measurements. In this protocol, we introduce a simple method for the reproducible extraction of metabolites, lipids and proteins from biological tissues using a single aliquot per sample. The extraction method is based on a methyl tert-butyl ether: methanol: water system for liquid: liquid partitioning of hydrophobic and polar metabolites into two immiscible phases along with the precipitation of proteins and other macromolecules as a solid pellet. This method, therefore, provides three different fractions of specific molecular composition, which are fully compatible with common high throughput 'omics' technologies such as liquid chromatography (LC) or gas chromatography (GC) coupled to mass spectrometers. Even though the method was initially developed for the analysis of different plant tissue samples, it has proved to be fully compatible for the extraction and analysis of biological samples from systems as diverse as algae, insects, and mammalian tissues and cell cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.