ArticlePloS one2017
Methods to maximise recovery of environmental DNA from water samples.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 325 citations in OpenAlex.
- Systematic review and meta-analysis: Water type and temperature affect environmental DNA decay.Molecular ecology resources · 2022Pooled it
- QuickConc Vacuum: A high-sensitive cationic-assisted method for large-volume eDNA concentration and sensitive biodiversity monitoring.MethodsX · 2026Article
- Protocols and challenges in managing multi-source biological evidence at the crime scene: a scoping review.Forensic science international. Synergy · 2026Review
- Optimizing Marine eDNA Protocols for Rare Megafauna: A Case Study on Dugongs in the Western Indian Ocean.Ecology and evolution · 2026Article
- Environmental DNA Metabarcoding Effectively Detects Invasive Species, Pests, and Community Changes in Taiwan's Rice Fields.Ecology and evolution · 2026Article
- Swab vs rollers: a comparison of methods to collect eDNA from vegetative surfaces.BMC research notes · 2026Article
- From Sequences to Food Webs: DNA Metabarcoding Reshapes Fish Trophic Ecology.Animals : an open access journal from MDPI · 2026Review
- Environmental DNA screening of Phocoenobacter atlanticus subsp. atlanticus in Atlantic salmon aquaculture.PloS one · 2026Article
- Optimization of environmental DNA-based methods: a case study for detecting brook trout (PeerJ · 2026Article
- Article
- Environmental DNA as a tool for hydropower impact assessments: current status, special considerations, and future integration.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Assessing Biodiversity at Eastern Oyster (Ecology and evolution · 2025Article
- Multi-Tool Marine Metabarcoding Bioassessment for Baselining and Monitoring Species and Communities in Kelp Habitats.Molecular ecology resources · 2025Article
- Methods, applications, and computational challenges in bait capture enrichment.Cell reports methods · 2025Review
- Ecological Health and Freshwater Pathogen Using eDNA Metabarcoding: A Preliminary Assessment for Environmental Surveillance Development in Malaysia.Microorganisms · 2025Article
- Metagenomic Assembled Genomes of aMicroorganisms · 2025Article
- Characterization of bacterial diversity associated with a Salar de Atacama native plant Nitrophila atacamensis.Environmental microbiome · 2025Article
- Waterborne Transmission Driving the Prevalence ofMicroorganisms · 2025Article
- Article
- Laboratory and In-Field Metagenomics for Environmental Monitoring.Methods in molecular biology (Clifton, N.J.) · 2025Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The environmental DNA (eDNA) method is a detection technique that is rapidly gaining credibility as a sensitive tool useful in the surveillance and monitoring of invasive and threatened species. Because eDNA analysis often deals with small quantities of short and degraded DNA fragments, methods that maximize eDNA recovery are required to increase detectability. In this study, we performed experiments at different stages of the eDNA analysis to show which combinations of methods give the best recovery rate for eDNA. Using Oriental weatherloach (Misgurnus anguillicaudatus) as a study species, we show that various combinations of DNA capture, preservation and extraction methods can significantly affect DNA yield. Filtration using cellulose nitrate filter paper preserved in ethanol or stored in a -20°C freezer and extracted with the Qiagen DNeasy kit outperformed other combinations in terms of cost and efficiency of DNA recovery. Our results support the recommendation to filter water samples within 24hours but if this is not possible, our results suggest that refrigeration may be a better option than freezing for short-term storage (i.e., 3-5 days). This information is useful in designing eDNA detection of low-density invasive or threatened species, where small variations in DNA recovery can signify the difference between detection success or failure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.