ArticlePloS one2017
Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Functional characterization of the second feedback loop in the circadian clock of the Antarctic krill Euphausia superba.BMC biology · 2024Article
- One Health: Circadian Medicine Benefits Both Non-human Animals and Humans Alike.Journal of biological rhythms · 2024Review
- Circadian miR-218-5p targets gene CA2 to regulate uterine carbonic anhydrase activity during egg shell calcification.Poultry science · 2022Article
- Evaluation of stable reference genes for qPCR normalization in circadian studies related to lung inflammation and injury in mouse model.Scientific reports · 2022Article
- Role of core circadian clock genes in hormone release and target tissue sensitivity in the reproductive axis.Molecular and cellular endocrinology · 2020Review
- Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions.BMC genomics · 2019Article
- Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors inFrontiers in physiology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The circadian clock is reported to play a role in the ovaries in a variety of vertebrate species, including the domestic hen. However, the ovary is an organ that changes daily, and the laying hen maintains a strict follicular hierarchy. The aim of this study was to examine the spatial-temporal expression of several known canonical clock genes in the granulosa and theca layers of six hierarchy follicles. We demonstrated that the granulosa cells (GCs) of the F1-F3 follicles harbored intrinsic oscillatory mechanisms in vivo. In addition, cultured granulosa cells (GCs) from F1 follicles exposed to luteinizing hormone (LH) synchronization displayed Per2 mRNA oscillations, whereas, the less mature GCs (F5 plus F6) displayed no circadian change in Per2 mRNA levels. Cultures containing follicle-stimulating hormone (FSH) combined with LH expressed levels of Per2 mRNA that were 2.5-fold higher than those in cultures with LH or FSH alone. These results show that there is spatial specificity in the localization of clock cells in hen preovulatory follicles. In addition, our results support the hypothesis that gonadotropins provide a cue for the development of the functional cellular clock in immature GCs.
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