ArticleNature cell biology2017
Pramel7 mediates ground-state pluripotency through proteasomal-epigenetic combined pathways.
Article in Nature cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- CtBP1/2 restrict DUX-dependent and independent 2C-like programs in mouse embryonic stem cells.EMBO reports · 2026Article
- PRAMEL12 orchestrates spermiogenesis to ensure male fertility in mice.The Journal of biological chemistry · 2026Article
- KIF2A stabilizes intercellular bridge microtubules to maintain mouse embryonic stem cell cytokinesis.The Journal of cell biology · 2025Article
- Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts.Nature communications · 2024Article
- Pramel15 facilitates zygotic nuclear DNMT1 degradation and DNA demethylation.Nature communications · 2024Article
- An RNA-dependent and phase-separated active subnuclear compartment safeguards repressive chromatin domains.Molecular cell · 2024Article
- TEAD2 initiates ground-state pluripotency by mediating chromatin looping.The EMBO journal · 2024Article
- Article
- Synergistic enhancement of the mouse Pramex1 and Pramel1 in repressing retinoic acid (RA) signaling during gametogenesis.Cell & bioscience · 2024Article
- An shRNA kinase screen identifies regulators of UHRF1 stability and activity in mouse embryonic stem cells.Epigenetics · 2022Article
- YY1 safeguard multidimensional epigenetic landscape associated with extended pluripotency.Nucleic acids research · 2022Article
- Identification ofGenes · 2022Article
- Involvement of PGC7 and UHRF1 in the regulation of DNA methylation of the IG-DMR in the imprintedActa biochimica et biophysica Sinica · 2022Article
- BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos.BMC biology · 2022Article
- Long non-coding RNA PCDRlnc1 confers docetaxel resistance in prostate cancer by promoting autophagy.Journal of Cancer · 2022Article
- Subcellular localization of the mouse PRAMEL1 and PRAMEX1 reveals multifaceted roles in the nucleus and cytoplasm of germ cells during spermatogenesis.Cell & bioscience · 2021Article
- Staying true to yourself: mechanisms of DNA methylation maintenance in mammals.Nucleic acids research · 2021Review
- Mthfd2 Modulates Mitochondrial Function and DNA Repair to Maintain the Pluripotency of Mouse Stem Cells.Stem cell reports · 2020Article
- A novel member of Prame family, Gm12794c, counteracts retinoic acid differentiation through the methyltransferase activity of PRC2.Cell death and differentiation · 2020Article
- Nucleolus and rRNA Gene Chromatin in Early Embryo Development.Trends in genetics : TIG · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
19 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naive pluripotency is established in preimplantation epiblast. Embryonic stem cells (ESCs) represent the immortalization of naive pluripotency. 2i culture has optimized this state, leading to a gene signature and DNA hypomethylation closely comparable to preimplantation epiblast, the developmental ground state. Here we show that Pramel7 (PRAME-like 7), a protein highly expressed in the inner cell mass (ICM) but expressed at low levels in ESCs, targets for proteasomal degradation UHRF1, a key factor for DNA methylation maintenance. Increasing Pramel7 expression in serum-cultured ESCs promotes a preimplantation epiblast-like gene signature, reduces UHRF1 levels and causes global DNA hypomethylation. Pramel7 is required for blastocyst formation and its forced expression locks ESCs in pluripotency. Pramel7/UHRF1 expression is mutually exclusive in ICMs whereas Pramel7-knockout embryos express high levels of UHRF1. Our data reveal an as-yet-unappreciated dynamic nature of DNA methylation through proteasome pathways and offer insights that might help to improve ESC culture to reproduce in vitro the in vivo ground-state pluripotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.