Evidence map›Paper›PMID 28603333›Full record

ArticleProceedings of SPIE--the International Society for Optical Engineering2017

16-Ch Time-resolved Single-Molecule Spectroscopy Using Line Excitation.

Antonino Ingargiola, Pietro Peronio, Eitan Lerner, Angelo Gulinatti, Ivan Rech, Massimo Ghioni, Shimon Weiss, Xavier Michalet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of SPIE--the International Society for Optical Engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. 16-Ch Time-resolved Single-Molecule Spectroscopy Using Line Excitation.Proceedings of SPIE--the International Society for Optical Engineering · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Antonino IngargiolaDept. Chemistry & Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Pietro PeronioDEIB, Politecnico di Milano, Milan, Italy.
Eitan LernerDept. Chemistry & Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Angelo GulinattiDEIB, Politecnico di Milano, Milan, Italy.
Ivan RechDEIB, Politecnico di Milano, Milan, Italy.
Massimo GhioniDEIB, Politecnico di Milano, Milan, Italy.
Shimon WeissDept. Chemistry & Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Xavier MichaletDept. Chemistry & Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.

Funding

Single Molecule Studies of Prokaryotic and Eukaryotic Transcription MachineriesR01GM069709 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2005 to 2012
$2.9M
Single-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0M
NIGMS NIH HHS R01 GM069709NIGMS NIH HHS R01 GM095904
6 · The paper itself

Abstract

Single-molecule spectroscopy on freely-diffusing molecules allows detecting conformational changes of biomolecules without perturbation from surface immobilization. Resolving fluorescence lifetimes increases the sensitivity in detecting conformational changes and overcomes artifacts common in intensity-based measurements. Common to all freely-diffusing techniques, however, are the long acquisition times. We report a time-resolved multispot system employing a 16-channel SPAD array and TCSPC electronics, which overcomes the throughput issue. Excitation is obtained by shaping a 532 nm pulsed laser into a line, matching the linear SPAD array geometry. We show that the line-excitation is a robust and cost-effective approach to implement multispot systems based on linear detector arrays.

Indexed as

fluorescencehigh-throughputsingle-moleculeSPAD arrayTCSPC

Identifiers

PMID28603333
PMCPMC5463578

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.