Evidence map›Paper›PMID 28603016›Full record

ArticleExperimental eye research2017

Rapid repeatable in vivo detection of retinal reactive oxygen species.

Ning Fan, Sean M Silverman, Yang Liu, Xizhen Wang, Byung-Jin Kim, Liping Tang, Abbot F Clark, Xuyang Liu, Iok-Hou Pang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 20 citations in OpenAlex.

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  12. mTORC1 Activation inOxidative medicine and cellular longevity · 2021
    Article
  13. Imaging Retinal Activity in the Living Eye.Annual review of vision science · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Ning FanShenzhen Eye Hospital, Shenzhen Key Laboratory of Ophthalmology, Jinan University, Shenzhen, China; North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA.
Sean M SilvermanNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA.
Yang LiuNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA.
Xizhen WangShenzhen Eye Hospital, Shenzhen Key Laboratory of Ophthalmology, Jinan University, Shenzhen, China.
Byung-Jin KimNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA.
Liping TangDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, USA.
Abbot F ClarkNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA.
Xuyang LiuShenzhen Eye Hospital, Shenzhen Key Laboratory of Ophthalmology, Jinan University, Shenzhen, China.
Iok-Hou PangNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Pharmaceutical Sciences, University of North Texas Health Science Center, Fort Worth, TX, USA. Electronic address: iok-hou.pang@unthsc.edu.
University of North Texas · USJinan University · CNThe University of Texas at Arlington · US

Funding

Non-invasive technique for in vivo detection of retinal reactive oxygen speciesR21EY024635 · NEI · UNIVERSITY OF NORTH TEXAS HLTH SCI CTR · PI PANG, IOK-HOU · 2014 to 2015
$398k
NEI NIH HHS R21 EY024635
6 · The paper itself

Abstract

Oxidative injuries, such as those related to reactive oxygen species (ROS), have been implicated in various retinal and optic nerve disorders. Many ROS detection methods have been developed. Although widely utilized, many of these methods are useful only in post mortem tissues, or require relatively expensive equipment, or involve intraocular injection. In the present study, we demonstrated and characterized a chemiluminescent probe L-012 as a noninvasive, in vivo ROS detection agent in the mouse retina. Using optic nerve crush (ONC) and retinal ischemia/reperfusion (I/R) as injury models, we show that L-012 produced intensive luminescent signals specifically in the injured eyes. Histological examination showed that L-012 administration was safe to the retina. Additionally, compounds that reduce tissue superoxide levels, apocynin and TEMPOL, decreased injury-induced L-012 chemiluminescence. The decrease in L-012 signals correlated with their protective effects against retinal I/R-induced morphological and functional changes in the retina. Together, these data demonstrate the feasibility of a fast, simple, reproducible, and non-invasive detection method to monitor in vivo ROS in the retina. Furthermore, the results also show that reduction of ROS is a potential therapeutic approach for protection from these retinal injuries.

Indexed as

AnimalsDisease Models, AnimalElectroretinographyFemaleLuminescent AgentsLuminolMiceMice, Inbred C57BLNerve CrushOptic Nerve InjuriesOxidative StressReactive Oxygen SpeciesReperfusion InjuryReproducibility of ResultsRetinal NeuronsL 012Luminescent AgentsLuminolReactive Oxygen SpeciesIschemiaL-012MouseOptic nerve injuryOxidative stressReactive oxidative speciesReperfusionRetina

Identifiers

PMID28603016
PMCPMC5554724
OpenAlexW2622730632

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.