Evidence map›Paper›PMID 28602889›Full record

ArticleJournal of neuroscience methods2017

Simple and reproducible approaches for the collection of select porcine ganglia.

David K Meyerholz, Leah R Reznikov

Open access · greenAbstract read
In one paragraph

Article in Journal of neuroscience methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Long-term culturing of porcine nodose ganglia.Journal of neuroscience methods · 2020
    Article
  4. Article
  5. Article
  6. The vagal ganglia transcriptome identifies candidate therapeutics for airway hyperreactivity.American journal of physiology. Lung cellular and molecular physiology · 2018
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

David K MeyerholzDepartment of Pathology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA. Electronic address: david-meyerholz@uiowa.edu.
Leah R ReznikovDepartment of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA. Electronic address: leahreznikov@ufl.edu.
University of Florida · USUniversity of Iowa · US

Funding

Vector CoreP01HL051670 · NHLBI · UNIVERSITY OF IOWA · PI MCCRAY, PAUL B · 1993 to 2019
$36.5M
Respiratory virus infection in early cystic fibrosis lung diseaseP01HL091842 · NHLBI · UNIVERSITY OF IOWA · PI ZABNER, JOSEPH · 2008 to 2022
$34.2M
Vector Core-Core 2P30DK054759 · NIDDK · UNIVERSITY OF IOWA · PI Amy Leanne Ryan · 1998 to 2026
$30.5M
Neural Pathogenesis of Airway Smooth Muscle Defects in Airway DiseaseR00HL119560 · NHLBI · UNIVERSITY OF FLORIDA · PI REZNIKOV, LEAH R · 2016 to 2018
$747k
NHLBI NIH HHS P01 HL051670NHLBI NIH HHS P01 HL091842NHLBI NIH HHS R00 HL119560NIDDK NIH HHS P30 DK054759
6 · The paper itself

Abstract

backgroundThe anatomy and physiology of the pig nervous system is more similar to humans compared to traditional rodent models. This makes the pig an attractive model to answer questions relating to human health and disease. Yet the technical and molecular tools available to pig researchers are limited compared to rodent researchers. NEW

methodWe developed simple and rapid methods to isolate the trigeminal, nodose (distal vagal), and dorsal root ganglia from neonatal pigs. We selected these ganglia due to their broad applicability to basic science researchers and clinicians.

resultsUse of these methods resulted in reproducible isolation of all three types of ganglia as validated by histological examination. COMPARISON WITH EXISTING METHOD(S): There are currently no methods that describe a step-by-step protocol to isolate these porcine ganglia.

conclusionsIn conclusion, these methods for ganglia collection will facilitate and accelerate future neuroscience investigations in pig models of human disease.

Indexed as

Ganglia, SpinalNodose GanglionSwineTrigeminal GanglionAnimalsAnimals, NewbornDissectionFemaleHistological TechniquesMaleDorsal root gangliaNodosePorcineTrigeminalVagal

Identifiers

PMID28602889
PMCPMC5582004
OpenAlexW2623956996

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.