ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2018
Histone methylation in the freeze-tolerant wood frog (Rana sylvatica).
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 50 citations in OpenAlex.
- Patterns of Local Adaptation in the Northern Leopard Frog (Evolutionary applications · 2026Article
- The transcriptomics and differential gene expression of freezing and thawing in a freeze-tolerant vertebrate.BMC genomics · 2026Article
- Molecular Mechanisms of DNA Damage Response and Epigenetic Regulation in Cold-Adapted Species: Implications for Genome Stability and Molecular Network Perspective.Current issues in molecular biology · 2025Review
- Contribution of reversible histone acetylation to freeze tolerance and recovery in wood frog kidneys.Scientific reports · 2025Article
- Epigenetic Regulation by Histone Methylation and Demethylation in Freeze-Tolerant Frog Kidney.Cell biochemistry and function · 2024Article
- The mRNA NAnimals : an open access journal from MDPI · 2024Article
- Histone Arginine Methylation as a Regulator of Gene Expression in the Dehydrating African Clawed Frog (Genes · 2024Article
- Reversible Histone Modifications Contribute to the Frozen and Thawed Recovery States of Wood Frog Brains.Biomolecules · 2024Article
- To live or let die? Epigenetic adaptations to climate change-a review.Environmental epigenetics · 2024Review
- Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall FlyInternational journal of molecular sciences · 2023Article
- DNA Hypomethylation May Contribute to Metabolic Recovery of Frozen Wood Frog Brains.Epigenomes · 2022Article
- Muscles in Winter: The Epigenetics of Metabolic Arrest.Epigenomes · 2021Review
- MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R.Molecular therapy. Nucleic acids · 2020Article
- Overwintering adaptations and extreme freeze tolerance in a subarctic population of the wood frog, Rana sylvatica.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2019Review
- Transcriptional regulation of metabolism in disease: From transcription factors to epigenetics.PeerJ · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freeze-tolerant animals survive sub-zero temperatures and long-term starvation associated with the winter by lowering their metabolic rate using a variety of transcriptional, translational, and post-translational regulatory methods. Histone methylation is one mechanism that is known to regulate gene expression at the transcriptional level. Here, we measured relative protein levels of seven histone methyltransferases (SMYD2, SETD7, ASH2L, RBBP5, SUV39H1, EHMT2, and SET8), four methylated histone H3 residues (H3K4me1, H3K9me3, H3K27me1, and H3K36me2), the methyltransferase activity on H3K4, and methylation of p53 (p53K370me2 and p53K372me1) in the skeletal muscle and liver of the freeze-tolerant wood frog (Rana sylvatica) during the freeze-thaw cycle. Overall, the results reveal a tissue-specific expression of histone methyltransferases and the methylation sites on histone H3 during freezing and thaw. In liver, H3K4me1 significantly decreased during freezing, H3K9me3 remained constant across conditions, H3K27me1 increased only during thaw, and H3K36me2 increased during freezing and then decreased during thaw (p < 0.05, n = 4). In skeletal muscle, H3K4me1 and H3K27me1 both decreased during freezing, whereas H3K9me3 and H3K36me2 were maintained across freezing and thaw (p < 0.05, n = 4). Methylation of p53 was also tissue-specific, where no changes were seen in liver tissue; however, p53 in skeletal muscle was differentially methylated. Overall, these results provide an evidence for the potential role methylation of histones and non-histone proteins play in freezing survival and entrance into a hypometabolic state.
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