Evidence map›Paper›PMID 28601321›Full record

ArticleThe Journal of surgical research2017

Lysophosphatidylcholine activates the Akt pathway to upregulate extracellular matrix protein production in human aortic valve cells.

Hui Cheng, Qingzhou Yao, Rui Song, Yufeng Zhai, Wei Wang, David A Fullerton, Xianzhong Meng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of surgical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Hui ChengDepartment of Surgery, University of Colorado Denver, Aurora, Colorado; Department of Cardiology, Shantou University Medical College, Shantou, China.
Qingzhou YaoDepartment of Surgery, University of Colorado Denver, Aurora, Colorado.
Rui SongDepartment of Surgery, University of Colorado Denver, Aurora, Colorado.
Yufeng ZhaiDepartment of Surgery, University of Colorado Denver, Aurora, Colorado.
Wei WangDepartment of Cardiology, Shantou University Medical College, Shantou, China.
David A FullertonDepartment of Surgery, University of Colorado Denver, Aurora, Colorado.
Xianzhong MengDepartment of Surgery, University of Colorado Denver, Aurora, Colorado. Electronic address: Xianzhong.meng@ucdenver.edu.
University of Colorado Denver · USShantou University · CN

Funding

Role of inflammatory response in aortic valve lesionsR01HL106582 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI MENG, XIANZHONG · 2011 to 2020
$3.8M
Suppression of AVIC inflammosteogenesis for prevention of CAVD progressionR01HL121776 · NHLBI · UNIVERSITY OF COLORADO DENVER · PI MENG, XIANZHONG · 2015 to 2023
$3.6M
NHLBI NIH HHS R01 HL106582NHLBI NIH HHS R01 HL121776
6 · The paper itself

Abstract

backgroundOverproduction of extracellular matrix (ECM) protein by aortic valve interstitial cells (AVICs) plays an important role in valvular sclerosis (thickening) associated with the early pathobiology of aortic stenosis. Accumulation of oxidized low-density lipoprotein (oxLDL) is observed in sclerotic aortic valve and may have a mechanistic role in valvular disease progression. Lysophosphatidylcholine (LysoPC) is a component of oxLDL and has multiple biological activities. This study was to test the hypothesis that oxLDL and LysoPC upregulate ECM protein production in human AVICs. METHODS AND

resultsAVICs were isolated from normal human aortic valves. Cells were treated with oxLDL (40 μg/mL) or LysoPC (40 μmol/L). Immunoblotting was applied to analyze ECM proteins (collagens I and III and biglycan) in cell lysate and Picrosirius red staining was used to examine collagen deposition. Both oxLDL and LysoPC upregulated the production of biglycan and collagen I. The upregulation of ECM proteins by LysoPC was preceded by the phosphorylation of Akt and ERK1/2. Inhibition of Akt markedly reduced the effect of LysoPC on ECM protein production and collagen deposition. However, inhibition of ERK1/2 had no effect.

conclusionsLysoPC upregulates the production of biglycan and collagen I in human AVICs and may mediate the effect of oxLDL on ECM protein production. The Akt pathway appears to be critical in mediating the effect of LysoPC. oxLDL accumulation and generation of LysoPC in the aortic valve tissue may contribute to the mechanism of valvular sclerosis associated with the development and progression of aortic stenosis.

Indexed as

Up-RegulationAgedAortic ValveAortic Valve StenosisBiglycanBiomarkersCells, CulturedCollagen Type ICollagen Type IIIExtracellular Matrix ProteinsFemaleHumansLipoproteins, LDLLysophosphatidylcholinesMaleMiddle AgedBiglycanBiomarkersCollagen Type ICollagen Type IIIExtracellular Matrix ProteinsLipoproteins, LDLLysophosphatidylcholinesoxidized low density lipoproteinProto-Oncogene Proteins c-aktAktAortic valveExtracellular matrix proteinLysophosphatidylcholineOxidized low-density lipoprotein

Identifiers

PMID28601321
PMCPMC5469546
OpenAlexW2592177877

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.