ArticleBMC infectious diseases2017
An ELISA assay using a combination of recombinant proteins from multiple strains of Orientia tsutsugamushi offers an accurate diagnosis for scrub typhus.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- The validity of diagnostic cut-offs for commercial and in-house scrub typhus IgM and IgG ELISAs: A review of the evidence.PLoS neglected tropical diseases · 2019Pooled it
- Neglected rickettsial diseases in Southeast Asia: Twenty-five years of progress in surveillance, diagnostics, and clinical research.PLoS neglected tropical diseases · 2026Review
- Review
- Development of a Novel Sandwich ELISA Test for the Detection of Antibodies Against Rickettsia.Pathogens (Basel, Switzerland) · 2025Article
- Estimating the Seroprevalence of Scrub Typhus in Nepal.Pathogens (Basel, Switzerland) · 2024Article
- Baculovirus-Assisted Production ofPathogens (Basel, Switzerland) · 2024Article
- Profile of Scrub Typhus Meningitis/Meningoencephalitis in Children with and without Scrub Typhus IgM Antibody in CSF.Journal of neurosciences in rural practice · 2021Article
- SARS-CoV-2 Serologic Assays in Control and Unknown Populations Demonstrate the Necessity of Virus Neutralization Testing.The Journal of infectious diseases · 2021Observational
- Development of a Scrub Typhus Diagnostic Platform Incorporating Cell-Surface Display Technology.Frontiers in immunology · 2021Article
- An Observational Study of Sepsis in Takeo Province Cambodia: An in-depth examination of pathogens causing severe infections.PLoS neglected tropical diseases · 2020Observational
- Determination of Optimal Diagnostic Cut-Offs for the Naval Medical Research Center Scrub Typhus IgM ELISA in Chiang Rai, Thailand.The American journal of tropical medicine and hygiene · 2019Article
- Scrub Typhus Complicated by ARDS, Myocarditis, and Encephalitis Imported to Oman from Nepal.Oman medical journal · 2019Article
- Detection and distribution of Sca autotransporter protein antigens in diverse isolates of Orientia tsutsugamushi.PLoS neglected tropical diseases · 2018Article
- Scrub Typhus: An Emerging Threat.Indian journal of dermatologyArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundScrub typhus (ST) is a disease caused by an obligate intracellular bacterium, Orientia tsutsugamushi, an organism that requires a BSL3 laboratory for propagation. The disease is hallmarked by an eschar at the site of the chigger bite, followed by the development of fever, malaise, myalgia, anorexia, and papulomacular rash. Indirect immunofluorescent assay (IFA) is the gold standard for scrub typhus diagnosis, however, the subjectivity of the assay, the need for a specialized laboratory and instruments has limited the wide use of the test in resource limited areas.
methodsA recombinant-protein based enzyme linked immunosorbent assay (ELISA) using the most abundant and immunodominant protein for the detection of Orientia specific antibodies in serum has been developed. The performance of the assay was evaluated using prospectively collected acute sera from 248 randomly selected patients in Thailand. The ELISA assay was evaluated using two different cutoff values.
resultsThe receiver operating characteristic (ROC) curve generated cutoff values gave slightly better consistency with diagnosis of ST than those cutoff values established by averaging ELISA optical density of known negatives at 99% confidence interval. Both cutoff values provided similar statistical parameters when compared with the diagnosis of ST, indicating the validity of both calculations to derive cutoff values. These results suggest that both IgG and IgM ELISA performed well to accurately diagnose scrub typhus cases in endemic areas using only acute serum samples.
conclusionsWe have successfully developed an ELISA assay for the detection of Orientia-specific antibodies in serum that could provide effective screening of acute sera under clinical setup and it is also a useful assay to estimate seroprevalence in various endemic areas.
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