Evidence map›Paper›PMID 28600982›Full record

ArticleRedox biology2017

Altered bioenergetics and enhanced resistance to oxidative stress in human retinal pigment epithelial cells from donors with age-related macular degeneration.

Deborah A Ferrington, Mara C Ebeling, Rebecca J Kapphahn, Marcia R Terluk, Cody R Fisher, Jorge R Polanco, Heidi Roehrich, Michaela M Leary, Zhaohui Geng, James R Dutton and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
132citing papers in PubMed, 1 pooled it
11.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

132 citing papers in PubMed, 1 synthesis or guideline pooled it, 186 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Retbindin Dynamically Redistributes to Preserve Retinal Flavin-Dependent Homeostasis in Response to Riboflavin Availability and Circadian Demand.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
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  7. Fluorescence lifetime imaging ophthalmoscopy (FLIO) of patients with neovascular age-related macular degeneration before and after treatment with intravitreal ranibizumab.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026
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72 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Deborah A FerringtonDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA; Graduate Program in Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: Ferri013@umn.edu.
Mara C EbelingDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: ebeli017@umn.edu.
Rebecca J KapphahnDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: kapph001@umn.edu.
Marcia R TerlukDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: mrterluk@umn.edu.
Cody R FisherDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA; Graduate Program in Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: fishe765@umn.edu.
Jorge R PolancoDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: polan070@umn.edu.
Heidi RoehrichHistology Core for Vision Research, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: rohri002@umn.edu.
Michaela M LearyDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: leary070@umn.edu.
Zhaohui GengStem Cell Institute and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: gengx027@umn.edu.
James R DuttonStem Cell Institute and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: dutto015@umn.edu.
Sandra R MontezumaDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: smontezu@umn.edu.
University of Minnesota · US

Funding

Visual TestingP30EY011374 · NEI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI NEWMAN, ERIC A · 1996 to 2019
$8.8M
Training Grant: Functional Proteomics of AgingT32AG029796 · NIA · UNIVERSITY OF MINNESOTA · PI EDGAR A ARRIAGA, Douglas G Mashek · 2008 to 2026
$6.6M
Age-related macular degeneration,mitochondria and the CFH genotypeR01EY026012 · NEI · UNIVERSITY OF MINNESOTA · PI FERRINGTON, DEBORAH ANN · 2016 to 2019
$1.7M
NEI NIH HHS P30 EY011374NEI NIH HHS R01 EY026012NIA NIH HHS T32 AG029796
6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is the leading cause of blindness among older adults. It has been suggested that mitochondrial defects in the retinal pigment epithelium (RPE) underlies AMD pathology. To test this idea, we developed primary cultures of RPE to ask whether RPE from donors with AMD differ in their metabolic profile compared with healthy age-matched donors. Analysis of gene expression, protein content, and RPE function showed that these cultured cells replicated many of the cardinal features of RPE in vivo. Using the Seahorse Extracellular Flux Analyzer to measure bioenergetics, we observed RPE from donors with AMD exhibited reduced mitochondrial and glycolytic function compared with healthy donors. RPE from AMD donors were also more resistant to oxidative inactivation of these two energy-producing pathways and were less susceptible to oxidation-induced cell death compared with cells from healthy donors. Investigation of the potential mechanism responsible for differences in bioenergetics and resistance to oxidative stress showed RPE from AMD donors had increased PGC1α protein as well as differential expression of multiple genes in response to an oxidative challenge. Based on our data, we propose that cultured RPE from donors phenotyped for the presence or absence of AMD provides an excellent model system for studying "AMD in a dish". Our results are consistent with the ideas that (i) a bioenergetics crisis in the RPE contributes to AMD pathology, and (ii) the diseased environment in vivo causes changes in the cellular profile that are retained in vitro.

Indexed as

Oxidative StressAgedAged, 80 and overCase-Control StudiesCells, CulturedEpithelial CellsFemaleGlycolysisHumansMacular DegenerationMaleMiddle AgedMitochondriaOxidative PhosphorylationPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaRetinal Pigment EpitheliumPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPPARGC1A protein, human6 max) Age-related macular degenerationAntioxidantsGlycolytic functionMitochondrial functionOxidative stressRetinal pigment epithelium

Identifiers

PMID28600982
PMCPMC5466586
OpenAlexW2620784367

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.