ArticleJournal of tissue engineering and regenerative medicine2018
Multi-layered silk film coculture system for human corneal epithelial and stromal stem cells.
Article in Journal of tissue engineering and regenerative medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Engineering anisotropic tissues: from structured scaffolds to magnetic actuation.Materials today. Bio · 2026Review
- Corneal Epithelial Tissue Engineering Strategy Based on Cell Viability Optimization: A Review and Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- From hard tissues to beyond: Progress and challenges of strontium-containing biomaterials in regenerative medicine applications.Bioactive materials · 2025Review
- Review
- Silk-Based Biomaterials for Designing Bioinspired Microarchitecture for Various Biomedical Applications.Biomimetics (Basel, Switzerland) · 2023Review
- Micropattern Silk Fibroin Film Facilitates Tendon RepairStem cells international · 2023Article
- Article
- Recent Advances in Natural Materials for Corneal Tissue Engineering.Bioengineering (Basel, Switzerland) · 2021Review
- Silk Film Stiffness Modulates Corneal Epithelial Cell Mechanosignaling.Macromolecular chemistry and physics · 2021Article
- The Human Tissue-Engineered Cornea (hTEC): Recent Progress.International journal of molecular sciences · 2021Review
- The Materiobiology of Silk: Exploring the Biophysical Influence of Silk Biomaterials on Directing Cellular Behaviors.Frontiers in bioengineering and biotechnology · 2021Review
- Fabrication and Biocompatibility of Electroconductive Silk Fibroin/PEDOT: PSS Composites for Corneal Epithelial Regeneration.Polymers · 2020Article
- In Vitro Culture of Human Corneal Endothelium on Non-Mulberry Silk Fibroin Films for Tissue Regeneration.Translational vision science & technology · 2020Article
- Engineering topography: Effects on corneal cell behavior and integration into corneal tissue engineering.Bioactive materials · 2019Review
- Corneal pain and experimental model development.Progress in retinal and eye research · 2019Review
- Scaffolding kidney organoids on silk.Journal of tissue engineering and regenerative medicine · 2019Article
- In Vitro Cultivation of Limbal Epithelial Stem Cells on Surface-Modified Crosslinked Collagen Scaffolds.Stem cells international · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
With insufficient options to meet the clinical demand for cornea transplants, one emerging area of emphasis is on cornea tissue engineering. In the present study, the goal was to combine the corneal stroma and epithelium into one coculture system, to monitor both human corneal stromal stem cell (hCSSC) and human corneal epithelial cell (hCE) growth and differentiation into keratocytes and differentiated epithelium in these three-dimensional tissue systems in vitro. Coculture conditions were first optimized, including the medium, air-liquid interface culture, and surface topography and chemistry of biomaterial scaffold films based on silk protein. The silk was used as scaffolding for both stromal and epithelial tissue layers because it is cell compatible, can be surface patterned, and is optically clear. Next, the effects of proliferating and differentiating hCEs and hCSSCs were studied in this in vitro system, including the effects on cell proliferation, matrix formation by immunochemistry, and gene expression by quantitative reverse transcription-polymerase chain reaction. The incorporation of both cell types into the coculture system demonstrated more complete differentiation and growth for both cell types compared to the corneal stromal cells and corneal epithelial cells alone. Silk films for corneal epithelial culture were optimized to combine a 4.0-μm-scale surface pattern with bulk-loaded collagen type IV. Differentiation of each cell type was in evidence based on increased expression of corneal stroma and epithelial proteins and transcript levels after 6 weeks in coculture on the optimized silk scaffolds.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.