Evidence map›Paper›PMID 28600315›Full record

ArticleApplied and environmental microbiology2017

RNA-Based Amplicon Sequencing Reveals Microbiota Development during Ripening of Artisanal versus Industrial Lard d'Arnad.

Ilario Ferrocino, Alberto Bellio, Angelo Romano, Guerrino Macori, Kalliopi Rantsiou, Lucia Decastelli, Luca Cocolin

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ilario FerrocinoDISAFA, Microbiology and Food Technology Sector, University of Turin, Grugliasco, Turin, Italy.
Alberto BellioSC Controllo Alimenti e Igiene delle Produzioni, Istituto Zooprofilattico Sperimentale PLV, Turin, Italy.
Angelo RomanoSC Controllo Alimenti e Igiene delle Produzioni, Istituto Zooprofilattico Sperimentale PLV, Turin, Italy.
Guerrino MacoriSC Controllo Alimenti e Igiene delle Produzioni, Istituto Zooprofilattico Sperimentale PLV, Turin, Italy.
Kalliopi RantsiouDISAFA, Microbiology and Food Technology Sector, University of Turin, Grugliasco, Turin, Italy.
Lucia DecastelliSC Controllo Alimenti e Igiene delle Produzioni, Istituto Zooprofilattico Sperimentale PLV, Turin, Italy.
Luca CocolinDISAFA, Microbiology and Food Technology Sector, University of Turin, Grugliasco, Turin, Italy lucasimone.cocolin@unito.it.
Istituto Zooprofilattico Sperimentale delle Venezie · ITUniversity of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Valle d'Aosta Lard d'Arnad is a protected designation of origin (PDO) product produced from fat of the shoulder and back of heavy pigs. Its manufacturing process can be very diverse, especially regarding the maturation temperature and the NaCl concentration used for the brine; thereby, the main goal of this study was to investigate the impact of those parameters on the microbiota developed during curing and ripening. Three farms producing Lard d'Arnad were selected. Two plants, reflecting the industrial process characterized either by low maturation temperature (plant A [10% NaCl, 2°C]) or by using a low NaCl concentration (plant B [2.5% NaCl, 4°C]), were selected, while the third was characterized by an artisanal process (plant C [30% NaCl, 8°C]). Lard samples were obtained at time 0 and after 7, 15, 30, 60, and 90 days of maturation. From each plant, 3 independent lots were analyzed. The diversity of live microbiota was evaluated by using classical plate counts and amplicon target sequencing of small subunit (SSU) rRNA. The main taxa identified by sequencing were

Indexed as

MicrobiotaAdipose TissueAnimalsBacteriaDietary FatsDNA, BacterialFood MicrobiologyPhylogenyRNA, RibosomalSwineDietary FatsDNA, BacteriallardRNA, RibosomalLard d'Arnadmeat live microbiotaRNA-based amplicon sequencingspoilage microbiota

Identifiers

PMID28600315
PMCPMC5541216
OpenAlexW2623321537

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.