ArticlePloS one2017
Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- Functional integration of an autologous engineered esophagus in a large-animal model.Nature biotechnology · 2026Article
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- Biochemical and mechanical effects of biopreservation methods on decellularized porcine skin grafts.BMC research notes · 2026Article
- Cryopreservation preserves the morpho-structural and mechanical integrity of human decellularized tracheas and supports a reduced immunogenic profile.Frontiers in bioengineering and biotechnology · 2026Article
- Decellularization Protocols for Esophagus Bioengineering: A Systematic Review.Bioengineering (Basel, Switzerland) · 2025Review
- Ultrasonic rewarming of cryopreserved alginate encapsulated liver spheroids.Scientific reports · 2025Article
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Development and qualification of clinical grade decellularized and cryopreserved human esophagi.Scientific reports · 2023Article
- The cell-assembled extracellular matrix: A focus on the storage stability and terminal sterilization of this human "bio" material.Acta biomaterialia · 2023Article
- Evaluation of Structural Viability of Porcine Tracheal Scaffolds after 3 and 6 Months of Storage under Three Different Protocols.Bioengineering (Basel, Switzerland) · 2023Article
- Regenerative medicine: current research and perspective in pediatric surgery.Pediatric surgery international · 2023Review
- Influence of Storage Conditions on Decellularized Porcine Conjunctiva.Bioengineering (Basel, Switzerland) · 2023Article
- Reconstruction of the human nipple-areolar complex: a tissue engineering approach.Frontiers in bioengineering and biotechnology · 2023Article
- Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022Review
- Lessons learned from pre-clinical testing of xenogeneic decellularized esophagi in a rabbit model.iScience · 2022Article
- Bio-Engineered Scaffolds Derived from Decellularized Human Esophagus for Functional Organ Reconstruction.Cells · 2022Article
- Building gut from scratch - progress and update of intestinal tissue engineering.Nature reviews. Gastroenterology & hepatology · 2022Review
- Application Progress of Modified Chitosan and Its Composite Biomaterials for Bone Tissue Engineering.International journal of molecular sciences · 2022Review
- Decellularized Colorectal Cancer Matrices as Bioactive Scaffolds for Studying Tumor-Stroma Interactions.Cancers · 2022Review
- The useful agent to have an ideal biological scaffold.Cell and tissue banking · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Oesophageal tissue engineering is a therapeutic alternative when oesophageal replacement is required. Decellularised scaffolds are ideal as they are derived from tissue-specific extracellular matrix and are non-immunogenic. However, appropriate preservation may significantly affect scaffold behaviour. Here we aim to prove that an effective method for short- and long-term preservation can be applied to tissue engineered products allowing their translation to clinical application. Rabbit oesophagi were decellularised using the detergent-enzymatic treatment (DET), a combination of deionised water, sodium deoxycholate and DNase-I. Samples were stored in phosphate-buffered saline solution at 4°C (4°C) or slow cooled in medium with 10% Me2SO at -1°C/min followed by storage in liquid nitrogen (SCM). Structural and functional analyses were performed prior to and after 2 and 4 weeks and 3 and 6 months of storage under each condition. Efficient decellularisation was achieved after 2 cycles of DET as determined with histology and DNA quantification, with preservation of the ECM. Only the SCM method, commonly used for cell storage, maintained the architecture and biomechanical properties of the scaffold up to 6 months. On the contrary, 4°C method was effective for short-term storage but led to a progressive distortion and degradation of the tissue architecture at the following time points. Efficient storage allows a timely use of decellularised oesophagi, essential for clinical translation. Here we describe that slow cooling with cryoprotectant solution in liquid nitrogen vapour leads to reliable long-term storage of decellularised oesophageal scaffolds for tissue engineering purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.