ArticleOrganogenesis2017
Enamel matrix derivative enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells on the titanium implant surface.
Article in Organogenesis, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Amelogenin enhances the reparative competence of trauma-affected periodontal ligament stem cells: implications for tooth autotransplantation.BMC oral health · 2026Article
- The Effect of Age and Use of Enamel Matrix Derivative on Implant Loss.Dentistry journal · 2026Article
- Graphene quantum dots remodel osteogenesis of human periodontal ligament stem cells under inflammatory microenvironment: anFrontiers in bioengineering and biotechnology · 2026Article
- Effects of injectable platelet-rich fibrin (i-PRF) application as root surface biomodification on gingival fibroblasts: an in vitro study.BMC oral health · 2025Article
- Research progress of enamel matrix derivative on periodontal tissue regeneration: a narrative review.Frontiers in dental medicine · 2025Review
- Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis.Scientific reports · 2023Article
- Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation.Medicina (Kaunas, Lithuania) · 2023Article
- Effects of CEM cement and emdogain on proliferation and differentiation of human stem cells from the apical papilla: a comparative in vitro study.Biotechnology letters · 2023Article
- Comparison of Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells through Application of Two β-tricalcium Phosphate Products: AnJournal of dentistry (Shiraz, Iran) · 2022Article
- Effect of biodentine coated with emdogain on proliferation and differentiation of human stem cells from the apical papilla.Molecular biology reports · 2022Article
- Enamel Matrix Derivative Enhances the Odontoblastic Differentiation of Dental Pulp Stem Cells via Activating MAPK Signaling Pathways.Stem cells international · 2022Article
- miR-141-3p Regulates EZH2 to Attenuate Porphyromonas gingivalis Lipopolysaccharide-Caused Inflammation and Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells.Computational and mathematical methods in medicine · 2022Article
- Modification of implant surfaces to stimulate mesenchymal cell activation.Bulletin of the National Research Centre · 2022Review
- Enamel Matrix Derivatives for Periodontal Regeneration: Recent Developments and Future Perspectives.Journal of healthcare engineering · 2022Review
- Deciphering the underlying mechanism of Xianlinggubao capsule against osteoporosis by network pharmacology.BMC complementary medicine and therapies · 2020Article
- Deciphering the Underlying Mechanism of Eucommiae Cortex against Osteoporotic Fracture by Network Pharmacology.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect.Journal of materials science. Materials in medicine · 2019Article
- circRNA CDR1as Regulated the Proliferation of Human Periodontal Ligament Stem Cells under a Lipopolysaccharide-Induced Inflammatory Condition.Mediators of inflammation · 2019Article
- Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Periodontal ligament stem cells (PDLSCs) have mesenchymal-stem-cells-like qualities, and are considered as one of the candidates of future clinical application in periodontal regeneration therapy. Enamel matrix derivative (EMD) is widely used in promoting periodontal regeneration. However, the effects of EMD on the proliferation and osteogenic differentiation of human PDLSCs grown on the Ti implant surface are still no clear. Therefore, this study examined the effects of EMD on human PDLSCs in vitro. Human PDLSCs were isolated from healthy participants, and seeded on the surface of Ti implant disks and stimulated with various concentrations of EMD. Cell proliferation was determined with Cell Counting Kit-8 (CCK-8). The osteogenic differentiation of PDLSCs was evaluated by the measurement of alkaline phosphatase (ALP) activity, Alizarin red staining, and real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. The results indicated that EMD at concentrations (5-60 µg/ml) increased the viability and proliferation of PDLSCs. The treatment with 30 and 60 µg/ml of EMD significantly elevated ALP activity, augmented mineralized nodule formation and calcium deposition, and upregulated the mRNA and protein levels of Runx-2 and osteocalcin (OCN) in the PDLSCs grown on the Ti surface. Further investigation found that EMD treatment did not change the protein levels of phosphatidylinositol-3-kinase (PI3K), p-PI3K, Akt and mTOR, but significantly upregulated the phosphorylated levels of Akt and mTOR. Collectively, these results suggest that EMD stimulation can promote the proliferation and osteogenic differentiation of PDLSCs grown on Ti surface, which is possibly associated with the activation of Akt/mTOR signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.