ArticleBotanical studies2016
Efficient and heritable transformation of Phalaenopsis orchids.
Article in Botanical studies, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Hybrid 2D-3D RGB-D Method for Growth Axis Estimation and Robotic Grasp Pose Generation of Phalaenopsis Orchid Buds.MethodsX · 2026Article
- Isolation and Identification ofMicroorganisms · 2026Article
- Efficient Regeneration and Genetic Transformation System for Cymbidium kanran 'Zhushalan'.Genes · 2026Article
- The Breeding, Cultivation, and Potential Applications of Ornamental Orchids with a Focus onPlants (Basel, Switzerland) · 2025Review
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Co-option of the SHOOT MERISTEMLESS network regulates protocorm-like body development in Phalaenopsis aphrodite.Plant physiology · 2022Article
- In-depth analysis of genomes and functional genomics of orchid using cutting-edge high-throughput sequencing.Frontiers in plant science · 2022Review
- Phalaenopsis orchid miniaturization by overexpression of OsGA2ox6, a rice GA2-oxidase gene.Botanical studies · 2020Article
- High-efficiency CRISPR/Cas-based editing of Phalaenopsis orchid MADS genes.Plant biotechnology journal · 2020Article
- Floral Induction and Flower Development of Orchids.Frontiers in plant science · 2019Review
- Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity.PLoS pathogens · 2018Article
- A Protoplast Transient Expression System to Enable Molecular, Cellular, and Functional Studies inFrontiers in plant science · 2018Article
- Building a Genetic Manipulation Tool Box for Orchid Biology: Identification of Constitutive Promoters and Application of CRISPR/Cas9 in the Orchid,Frontiers in plant science · 2016Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundPhalaenopsis orchid (Phal. orchid) is visually attractive and it is important economic floriculture species. Phal. orchids have many unique biological features. However, investigation of these features and validation on their biological functions are limited due to the lack of an efficient transformation method.
resultsWe developed a heritable and efficient Agrobacterium- mediated transformation using protocorms derived from tetraploid or diploid Phal. orchids. A T-DNA vector construct containing eGFP driven by ubiquitin promoter was subjected to transformation. An approximate 1.2-5.2 % transformation rate was achieved. Genomic PCR confirmed that hygromycin selection marker, HptII gene and target gene eGFP were integrated into the orchid genome. Southern blotting indicated a low T-DNA insertion number in the orchid genome of the transformants. Western blot confirmed the expression of eGFP protein in the transgenic orchids. Furthermore, the GFP signal was detected in the transgenic orchids under microscopy. After backcrossing the pollinia of the transgenic plants to four different Phal. orchid varieties, the BC1 progenies showed hygromycin resistance and all surviving BC1 seedlings were HptII positive in PCR and expressed GFP protein as shown by western blot.
conclusionsThis study demonstrated a stable transformation system was generated for Phal. orchids. This useful transformation protocol enables functional genomics studies and molecular breeding.
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