Evidence map›Paper›PMID 28597195›Full record

ArticleVirus genes2017

Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology.

Heng Wang, Rongchang Liu, Weidong Zhang, Lingshuang Sun, Zhangyong Ning, Fangxiao Ji, Jin Cui, Guihong Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Virus genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. H1N1 influenza virus epitopes classified by monoclonal antibodies.Experimental and therapeutic medicine · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Heng WangCollege of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
Rongchang LiuInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, Fujian Province, China.
Weidong ZhangHospital of South China Agricultural University, Guangzhou, 510642, Guangdong Province, China.
Lingshuang SunCollege of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
Zhangyong NingCollege of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China.
Fangxiao JiMOA Key Laboratory of Animal Vaccine Development, Guangzhou, 510642, Guangdong Province, China.
Jin CuiMOA Key Laboratory of Animal Vaccine Development, Guangzhou, 510642, Guangdong Province, China.
Guihong ZhangCollege of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, 510642, Guangdong Province, China. guihongzh@scau.edu.cn.
South China Agricultural University · CNKey Laboratory of Guangdong Province · CNFujian Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nonstructural protein 7 (nsp7) of porcine reproductive and respiratory syndrome virus (PRRSV) is considered to be a suitable reagent for the development of serological diagnostic assays. It can be expressed as a soluble recombinant protein in Escherichia coli, and its antibody response may continue up to 202 days post-infection. Furthermore, the region encoded by nsp7 is highly homologous among various strains within the genotype, and the results of nsp7-based enzyme-linked immunosorbent assay (ELISA) showed high agreement with previous Idexx ELISA results. All these evidences suggest the existence of important epitopes on nsp7, though the characteristics of these epitopes remain unclear. In the present study, we prepared three monoclonal antibodies against nsp7 protein and used them to screen the epitope-distribution characteristics of PRRSV nsp7 protein by phage-display technology. We identified a linear epitope NAWGDEDRLN at amino acids 153-162 type II PRRSV nsp7β subunit. This newly defined epitope showed excellent reactivity with PRSSV-positive serum samples. These results further our understanding of the antigenic structure of nsp7 protein, and provide efficient reagents for PRRSV serological tests.

Indexed as

Amino Acid MotifsAnimalsAntibodies, MonoclonalAntibodies, ViralBacteriophagesEnzyme-Linked Immunosorbent AssayEpitopesPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSwineViral Nonstructural ProteinsAntibodies, MonoclonalAntibodies, ViralEpitopesViral Nonstructural ProteinsAntigenic structureEpitopesNonstructural protein 7Phage-display technologyPorcine reproductive and respiratory syndrome virus

Identifiers

PMID28597195
OpenAlexW2624582564

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.