Evidence map›Paper›PMID 28597192›Full record

ArticleJapanese journal of ophthalmology2017

Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty.

Takenori Inomata, Koichi Ono, Tsuyoshi Matsuba, Tina Shiang, Antonio Di Zazzo, Satoru Nakatani, Masahiro Yamaguchi, Nobuyuki Ebihara, Akira Murakami

Abstract read
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In one paragraph

Article in Japanese journal of ophthalmology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Trial
  2. Article
  3. Intensive care unit time and prolonged enucleation to processing interval are associated with donor cornea contamination.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Takenori InomataDepartment of Ophthalmology, Juntendo University Faculty of Medicine, Bunkyo-ku, 2-1-1 Hongo, Tokyo, 113-0033, Japan. tinoma@juntendo.ac.jp.
Koichi OnoDepartment of Ophthalmology, Juntendo Tokyo Koto Geriatric Medical Center, Juntendo University School of Medicine, Koto-ku, Tokyo, Japan.
Tsuyoshi MatsubaAdvanced Research Center for Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.
Tina ShiangDepartment of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA.
Antonio Di ZazzoBioMolecular and Cellular Laboratories in Ophthalmology, IRCCS, G.B. Bietti Foundation, Rome, Italy.
Satoru NakataniDepartment of Ophthalmology, Juntendo University Faculty of Medicine, Bunkyo-ku, 2-1-1 Hongo, Tokyo, 113-0033, Japan.
Masahiro YamaguchiDepartment of Ophthalmology, Juntendo University Faculty of Medicine, Bunkyo-ku, 2-1-1 Hongo, Tokyo, 113-0033, Japan.
Nobuyuki EbiharaDepartment of Ophthalmology, Juntendo University Urayasu Hospital, Tomioka, Chiba, Japan.
Akira MurakamiDepartment of Ophthalmology, Juntendo University Faculty of Medicine, Bunkyo-ku, 2-1-1 Hongo, Tokyo, 113-0033, Japan.
Juntendo University · JPFondazione G.B. Bietti · ITJuntendo University Urayasu Hospital · JPMassachusetts Eye and Ear Infirmary · USWaseda University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (Optisol™-GS) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in Optisol™-GS by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.

methodsWe conducted a retrospective, cross-sectional study using Juntendo Eye Bank records for all corneal transplantations. Two hundred donor conjunctiva harvestings and storage medium (EP-II

resultsWe obtained positive bacterial cultures from 154 of the 200 eyes (77.0%). The isolated bacteria were indigenous, such as coagulase-negative Staphylococci, Corynebacterium sp., and methicillin-resistant Staphylococcus aureus (MRSA). There was significant resistance to levofloxacin (18 eyes, 9.0%) and gentamicin (12 eyes, 6.0%), and no vancomycin-resistant bacteria were detected. The donor factors did not correlate with the prevalence of bacterial contamination in our criteria.

conclusionsPre-banking microbial assessment allows for microbial detection, bacterial susceptibility and resistance testing. This is useful for developing preservation mediums containing effective spectrum antibiotic agents for high quality control of corneal banking.

Indexed as

Eye BanksKeratoplasty, PenetratingOrgan PreservationAdultAgedAged, 80 and overBacteriaChondroitin SulfatesComplex MixturesConjunctivaCross-Sectional StudiesCulture Media, Serum-FreeDextransEye Infections, BacterialFemaleGentamicinsChondroitin SulfatesComplex MixturesCulture Media, Serum-FreeDextransGentamicinsOptisolBacterial contaminationCorneoscleral rimDonor conjunctivaEye bankingPreservation medium

Identifiers

PMID28597192
OpenAlexW2623739613

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.