ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2017
Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- NRF2 activation in cancer cells suppresses immune infiltration into the tumor microenvironment.iScience · 2025Article
- Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system.Odontology · 2024Article
- Functions of Hemp-Induced Exosomes against Periodontal Deterioration Caused by Fine Dust.International journal of molecular sciences · 2024Article
- Review
- Salivary secretory leukocyte protease inhibitor levels in patients with stage 3 grade C periodontitis: a comparative cross-sectional study.Scientific reports · 2022Article
- Single Cell RNA Sequencing Reveals Critical Functions ofFrontiers in cell and developmental biology · 2022Article
- The Role of Recombinant Secretory Leukocyte Protease Inhibitor to CD163, FGF-2, IL-1 and IL-6 Expression in Skin Wound Healing.Clinical, cosmetic and investigational dermatology · 2022Article
- Mechanisms involved in regulation of periodontal ligament cell production of pro-inflammatory cytokines: Implications in periodontitis.Journal of periodontal research · 2021Review
- For Better or for Worse: A Look Into Neutrophils in Traumatic Spinal Cord Injury.Frontiers in cellular neuroscience · 2021Review
- The Inhibition of Inflammatory Signaling Pathway by Secretory Leukocyte Protease Inhibitor can Improve Spinal Cord Injury.Cellular and molecular neurobiology · 2020Review
- CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues-in vitro and in vivo studies.Clinical oral investigations · 2020Article
- Investigating unset endodontic sealers' eugenol and hydrocortisone roles in modulating the initial steps of inflammation.Clinical oral investigations · 2020Article
- Effects of compound lobelia oral liquid on acute pharyngitis rabbits model.Saudi journal of biological sciences · 2019Article
- Identification of novel mRNAs and lncRNAs associated with mouse experimental colitis and human inflammatory bowel disease.American journal of physiology. Gastrointestinal and liver physiology · 2018Article
- Identification of Secretory Leukoprotease Inhibitor As an Endogenous Negative Regulator in Allergic Effector Cells.Frontiers in immunology · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveRegulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. MATERIALS AND
methodsPDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression.
resultsPDL cells showed cytoplasmic expression of SLPI. Cellular expression level of SLPI negatively correlated to LPS-induced stimulation of IL-6 and MCP-1. Both SLPI gene activity and protein were reduced by about 70% in PDL cells treated with SLPI siRNA compared to cells treated with non-coding construct. Treatment with SLPI siRNA was associated with up-regulation of both basal and LPS-stimulated IL-6, MCP-1 and TLRs mRNA expression. The up-regulation of MCP-1 transcript in SLPI siRNA-treated cells was confirmed on protein level. SLPI siRNA-treatment enhanced the phosphorylated NF-κB p105 protein expression.
conclusionsSLPI regulates PDL cell pro-inflammatory cytokine expression and modulates NF-κB signaling, suggesting that SLPI governs the immune cell-like properties of PDL cells.
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