ArticleScientific reports2017
Development of an efficient vector system for gene knock-out and near in-cis gene complementation in the sugarcane smut fungus.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- A Genetically EngineeredMicroorganisms · 2023Article
- SsUbc2, a determinant of pathogenicity, functions as a key coordinator controlling global transcriptomic reprogramming during mating in sugarcane smut fungus.Frontiers in microbiology · 2022Article
- SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus.Journal of fungi (Basel, Switzerland) · 2021Article
- Genetic Manipulation of the Brassicaceae Smut FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Applications of CRISPR/Cas9 in the Synthesis of Secondary Metabolites in Filamentous Fungi.Frontiers in microbiology · 2021Review
- Advances in targeting and heterologous expression of genes involved in the synthesis of fungal secondary metabolites.RSC advances · 2019Review
- Progress and Challenges: Development and Implementation of CRISPR/Cas9 Technology in Filamentous Fungi.Computational and structural biotechnology journal · 2019Review
- Identification and Functional Analysis of the Pheromone Response Factor Gene ofFrontiers in microbiology · 2019Article
- The Farnesyltransferase β-Subunit Ram1 RegulatesFrontiers in microbiology · 2019Article
- Development of an Expression Vector to Overexpress or Downregulate Genes inJournal of fungi (Basel, Switzerland) · 2018Article
- The MAP Kinase SsKpp2 Is Required for Mating/Filamentation inFrontiers in microbiology · 2018Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sporisorium scitamineum is the causative agent responsible for smut disease of sugarcane worldwide. However, lack of efficient gene manipulation system makes this fungus much behind the type model of the smut fungi in molecular biology. Here, we report the development of a CRISPR/Cas9 and T-DNA based dual vector system that allowed efficient knock-out or knock-in of a gene of interest in the S. scitamineum in a site-specific manner. By using Mfa2, a key player in the mating event in S. scitamineum as a tester gene, site-specific insertions of the introduced fragments were achieved both for Mfa2 knockout and complementation. Of particular advantage of this system is the simplicity of selection and identification for the desired transformants by using drug resistance coupled with PCR. This system greatly facilitates the gene function study in S. scitamineum, and could potentially be used for other basidiomycete fungi.
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