Evidence map›Paper›PMID 28596239›Full record

ArticleJournal of cell science2017

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Yu-Ja Huang, Paula Schiapparelli, Kristen Kozielski, Jordan Green, Emily Lavell, Hugo Guerrero-Cazares, Alfredo Quinones-Hinojosa, Peter Searson

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yu-Ja HuangInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Paula SchiapparelliDepartment of Neurosurgery and Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21231, USA.
Kristen KozielskiInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Jordan GreenInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Emily LavellDepartment of Neurosurgery and Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21231, USA.
Hugo Guerrero-CazaresInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
Alfredo Quinones-HinojosaDepartment of Neurosurgery and Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD 21231, USA.
Peter SearsonInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD 21218, USA searson@jhu.edu.ORCID http://orcid.org/0000-0002-5417-0828
Johns Hopkins University · US

Funding

Role of NKCC1 on Brain Tumor Stem Cell Migration After EGF and Slit-2 StimulationR01NS070024 · NINDS · JOHNS HOPKINS UNIVERSITY · PI QUINONES-HINOJOSA, ALFREDO · 2010 to 2014
$2.4M
An engineered platform for the study of metastasis (PQ #24)R01CA170629 · NCI · JOHNS HOPKINS UNIVERSITY · PI SEARSON, PETER C · 2012 to 2015
$1.3M
Role of Human Cerebrospinal Fluid Chemokines in the Invasiveness of Human GlioblastomaR21CA199295 · NCI · MAYO CLINIC JACKSONVILLE · PI GUERRERO-CAZARES, HUGO · 2016 to 2017
$384k
NCI NIH HHS R01 CA170629NINDS NIH HHS R01 NS070024
6 · The paper itself

Abstract

Endogenous electric fields modulate many physiological processes by promoting directional migration, a process known as galvanotaxis. Despite the importance of galvanotaxis in development and disease, the mechanism by which cells sense and migrate directionally in an electric field remains unknown. Here, we show that electrophoresis of cell surface heparan sulfate (HS) critically regulates this process. HS was found to be localized at the anode-facing side in fetal neural progenitor cells (fNPCs), fNPC-derived astrocytes and brain tumor-initiating cells (BTICs), regardless of their direction of galvanotaxis. Enzymatic removal of HS and other sulfated glycosaminoglycans significantly abolished or reversed the cathodic response seen in fNPCs and BTICs. Furthermore, Slit2, a chemorepulsive ligand, was identified to be colocalized with HS in forming a ligand gradient across cellular membranes. Using both imaging and genetic modification, we propose a novel mechanism for galvanotaxis in which electrophoretic localization of HS establishes cell polarity by functioning as a co-receptor and provides repulsive guidance through Slit-Robo signaling.

Indexed as

Cell MovementElectrophoresisCell Line, TumorCell MembraneHeparan SulfateHumansNeurogliaHeparan SulfateBrain tumor-initiating cellsElectrophoresisGalvanotaxisHeparan sulfate

Identifiers

PMID28596239
PMCPMC5558272
OpenAlexW2622272699

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.