Evidence map›Paper›PMID 28595966›Full record

ArticleAnalytical biochemistry2017

Characterization of PKACα enzyme kinetics and inhibition in an HPLC assay with a chromophoric substrate.

Nicole M Luzi, Charles E Lyons, Darrell L Peterson, Keith C Ellis

Abstract read
In one paragraph

Article in Analytical biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nicole M LuziDepartment of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298-0540, United States.
Charles E LyonsMassey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298-0035, United States.
Darrell L PetersonInstitute for Structural Biology, Drug Discovery, and Development, Virginia Commonwealth University, Richmond, VA 23219-1540, United States; Department of Biochemistry and Molecular Biology, School of Medicine, Virginia Commonwealth University, Richmond, VA 23298-0614, United States.
Keith C EllisDepartment of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, VA 23298-0540, United States; Massey Cancer Center, Virginia Commonwealth University, Richmond, VA 23298-0035, United States; Institute for Structural Biology, Drug Discovery, and Development, Virginia Commonwealth University, Richmond, VA 23219-1540, United States. Electronic address: kcellis@vcu.edu.

Funding

Virus Vector Shared ResourceP30CA016059 · NCI · VIRGINIA COMMONWEALTH UNIVERSITY · PI Keri R Maher · 1985 to 2026
$51.0M
NCI NIH HHS P30 CA016059
6 · The paper itself

Abstract

Here we describe a convenient, inexpensive, and non-hazardous method for the measurement of the kinase activity of the catalytic subunit of cAMP-dependent protein kinase (PKACα). The assay is based on the separation of a substrate peptide labeled with a strong chromophore from the phosphorylated product peptide by high-performance liquid chromatograph (HPLC) and quantification of the product ratiometrically at a wavelength in the visual spectrum (Vis). The utility and reliability of the HPLC-Vis assay were demonstrated by characterizing the kinetic parameters (K

Indexed as

Biological AssayCatalytic DomainChromatography, High Pressure LiquidCyclic AMP-Dependent Protein KinasesHumansKineticsOligopeptidesPhosphorylationProtein Kinase InhibitorsReproducibility of ResultsSubstrate SpecificityCyclic AMP-Dependent Protein KinaseskemptideOligopeptidesProtein Kinase InhibitorscAMP-dependent protein kinaseChromophoreHPLCInhibition assayKinase assayKinetics assay

Identifiers

PMID28595966
PMCPMC5889107

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.