ArticleBMC genomics2017
Assembly and use of high-density recombinant peptide chips for large-scale ligand screening is a practical alternative to synthetic peptide libraries.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- EpiScan: accurate high-throughput mapping of antibody-specific epitopes using sequence information.NPJ systems biology and applications · 2024Article
- Self-Assembled Peptide-Labeled Probes for Agglutination-Based Sensing.Macromolecular research · 2021Article
- A Peptide-Lectin Fusion Strategy for Developing a Glycan Probe for Use in Various Assay Formats.Chemosensors (Basel, Switzerland) · 2019Article
- Combinatorial Peptide Microarray Synthesis Based on Microfluidic Impact Printing.ACS combinatorial science · 2019Article
- IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.Chemosensors (Basel, Switzerland) · 2018Article
- Peptide Arrays: Development and Application.Analytical chemistry · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecombinant peptide chips could constitute a versatile complementation to state-of-the-art in situ (chemical on-chip) synthesis, particle-based printing, or pre-manufactured peptide spotting. Bottlenecks still impeding a routine implementation - from restricted peptide lengths, low diversity and low array densities to high costs - could so be overcome.
methodsTo assess overall performance, we assembled recombinant chips composed of 38,400 individual peptide spots on the area of a standard 96-well microtiter plate from comprehensive, highly diverse (>107 single clones) short random peptide libraries.
resultsScreening of altogether 476,160 clones against Streptavidin uncovered 2 discrete new binders: a characteristic HPQ-motif containing VSHPQAPF and a cyclic CSGSYGSC peptide. Interactions were technically confirmed by fluorescence polarization as well as biolayer-interferometry, and their potential suitability as novel detection tags evaluated by detection of a peptide-fused exemplary test protein.
conclusionFrom our data we conclude that the presented technical pipeline can reliably identify novel hits, useful as first-generation binders or templates for subsequent ligand design plus engineering.
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