ArticleBMC infectious diseases2017
Multiplex detection in tonsillar tissue of all known human polyomaviruses.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Absence of DNA viruses in ameloblastomas.European journal of oral sciences · 2026Article
- Prevalence and in vitro study of human polyomavirus 9.Scientific reports · 2024Article
- Viral DNA in submandibular gland tissue with an inflammatory disorder.Journal of oral microbiology · 2024Article
- Unmasking the tissue-resident eukaryotic DNA virome in humans.Nucleic acids research · 2023Article
- Emerging role of human polyomaviruses 6 and 7 in human cancers.Infectious agents and cancer · 2021Review
- Detection of Human polyomavirus 2 (HPyV2) in oyster samples in northern Brazil.Virology journal · 2020Article
- Article
- Newly detected DNA viruses in juvenile nasopharyngeal angiofibroma (JNA) and oral and oropharyngeal squamous cell carcinoma (OSCC/OPSCC).European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2019Article
- The occurrence of polyomaviruses WUPyV and KIPyV among patients with severe respiratory infections.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Antibody response to polyomavirus primary infection: high seroprevalence of Merkel cell polyomavirus and lymphoid tissue involvement.Journal of neurovirology · 2018Article
- Polyomaviruses detectable in head and neck carcinomas.Oncotarget · 2018Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the past few years, eleven new human viruses have joined the two previously known members JCPyV and BKPyV of the Polyomaviridae family, by virtue of molecular methods. Serology data suggest that infections with human polyomaviruses (HPyVs) occur since childhood and the viruses are widespread in the general population. However, the viral persistence sites and transmission routes are by and large unknown. Our previous studies demonstrated that the four new HPyVs - KIPyV, WUPyV, MCPyV and TSPyV - were present in the tonsils, and suggested lymphoid tissue as a persistent site of these emerging human viruses. We developed a Luminex-based multiplex assay for simultaneous detection of all 13 HPyVs known, and explored their occurrence in tonsillar tissues of children and adults mostly with tonsillitis or tonsillar hypertrophy.
methodsWe set up and validated a new Luminex-based multiplex assay by using primer pairs and probes targeting the respective HPyV viral protein 1 (VP1) genes. With this assay we tested 78 tonsillar tissues for DNAs of 13 HPyVs.
resultsThe multiplex assay allowed for simultaneous detection of 13 HPyVs with high analytical sensitivity and specificity, with detection limits of 10
conclusionsThe Luminex-based HPyV multiplex assay appears highly suitable for clinical diagnostic purposes and large-scale epidemiological studies. Additional evidence was acquired that the lymphoid system plays a role in HPyV infection and persistence. Thereby, shedding from this site during reactivation might take part in transmission of the newly found HPyVs.
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