Evidence map›Paper›PMID 28594266›Full record

ArticleBioengineered2017

Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe.

Changbei Ma, Xiaoyuan Lv, Kemin Wang, Shunxin Jin, Haisheng Liu, Kefeng Wu, Weimin Zeng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineered, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Changbei Maa State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.
Xiaoyuan Lvb State Key Laboratory of Chemo/Biosensing and Chemometrics , Hunan University , Changsha , China.
Kemin Wangb State Key Laboratory of Chemo/Biosensing and Chemometrics , Hunan University , Changsha , China.
Shunxin Jina State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.
Haisheng Liua State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.
Kefeng Wua State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.
Weimin Zenga State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.
Central South University · CNHunan University · CNState Key Laboratory of Chemobiosensing and Chemometrics

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein kinase A was detected by quantifying the amount of ATP used after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. The ligation product then hybridized to MB, resulting in a fluorescence enhancement of the MB. This assay was capable of determining protein kinase A in the range of 12.5∼150 nM, with a detection limit of 1.25 nM. Furthermore, this assay could also be used to investigate the effect of genistein on protein kinase A. It was a universal, non-radioisotopic, and homogeneous method for assaying protein kinase A.

Indexed as

Adenosine TriphosphateCyclic AMP-Dependent Protein KinasesDNADNA LigasesEnzyme AssaysFluorescenceMolecular ProbesPhosphorylationAdenosine TriphosphateCyclic AMP-Dependent Protein KinasesDNADNA LigasesMolecular ProbesATPDNA ligaseinhibitormolecular beaconprotein kinase A

Identifiers

PMID28594266
PMCPMC5736335
OpenAlexW2622294129

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.