ArticleJournal of biological engineering2017
Streamlining recombination-mediated genetic engineering by validating three neutral integration sites in
Article in Journal of biological engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression.Current issues in molecular biology · 2026Review
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- Astaxanthin Overproduction Enhanced by Metabolomics-Guided Rational Metabolic Engineering inACS synthetic biology · 2025Article
- Improving the Transformation Efficiency ofACS synthetic biology · 2025Article
- Food Webs and Feedbacks: The Untold Ecological Relevance of Antimicrobial Resistance as Seen in Harmful Algal Blooms.Microorganisms · 2024Review
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Exploring the Potential of the Model CyanobacteriaBiomolecules · 2023Article
- Development of a Biotechnology Platform for the Fast-Growing CyanobacteriumBiomolecules · 2022Article
- Insights into cyanobacterial alkane biosynthesis.Journal of industrial microbiology & biotechnology · 2022Review
- Synthetic biology in marine cyanobacteria: Advances and challenges.Frontiers in microbiology · 2022Review
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Computational Analysis of Dynamic Light Exposure of Unicellular Algal Cells in a Flat-Panel Photobioreactor to Support Light-Induced COFrontiers in microbiology · 2021Article
- From the Ocean to the Lab-Assessing Iron Limitation in Cyanobacteria: An Interface Paper.Microorganisms · 2020Review
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
- CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax.Plant physiology · 2019Article
- New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering.Frontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background
resultsWe designed three BioBrick-compatible genetic modules, each targeting a separate neutral integration site, A2842, A0935, and A0159, with varying length of homologous region, spanning from 100 to 800 nt. The performance of the different modules for achieving DNA integration were tested. Our results demonstrate that 100 nt homologous regions are sufficient for inserting a 1 kb DNA fragment into the
conclusionsThe optimized transformation protocol reported in this study provides an efficient way to perform genetic engineering in
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Registered trials
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