Evidence map›Paper›PMID 28592924›Full record

ArticleCancer cell international2017

Suppression of endothelial cell migration by tumor associated macrophage-derived exosomes is reversed by epithelial ovarian cancer exosomal lncRNA.

Quanfeng Wu, Xiaoli Wu, Xiang Ying, Qinyi Zhu, Xinjing Wang, Lu Jiang, Xin Chen, Yueqian Wu, Xipeng Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed, 2 pooled it
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 2 syntheses or guidelines pooled it, 104 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Review
  6. Exosomes in Ovarian Cancer: Towards Precision Oncology.Pharmaceuticals (Basel, Switzerland) · 2025
    Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Exosomal Cargo in Ovarian Cancer Dissemination.Current issues in molecular biology · 2023
    Review
  13. Review
  14. Exosome Mediated Cancer Therapeutic Approach:Present Status and Future Prospectives.Asian Pacific journal of cancer prevention : APJCP · 2023
    Review
  15. Review
  16. Review
  17. Review
  18. Article
  19. Review
  20. Review

1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Quanfeng Wu *Department of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiaoli Wu *Department of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiang YingDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Qinyi ZhuDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xinjing WangDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lu JiangDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xin ChenDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yueqian WuDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xipeng WangDepartment of gynaecology and obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
XinHua Hospital · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo study the mechanism by which epithelial ovarian cancer (EOC)-derived exosomes restore the migration of endothelial cells that is suppressed by TAM-derived exosomes.

methodsExosomes were isolated from TAMs in the ascites of patients with EOC. The effect of exosomes on the expression of endothelial cell miRNA was monitored by PCR. The miRNA mimics were transfected to explore their effects. Microarray data and literature searches were used to predict target genes and the impact of target gene pathways, and small interfering RNA was used to target these genes. We used migration assays to determine whether ovarian cancer cell-derived exosomes participate in the regulation of TAMs and endothelial cells. We used microarray data to identify the target lncRNA, and we constructed target lncRNA expression plasmids to validate targets by Western blotting.

resultsWe separated TAMs from the ascites of patients with EOC and isolated exosomes from TAM supernatants. After co-culture with HUVECs, these exosomes were efficiently incorporated into HUVECs. The migration of HUVECs was suppressed significantly in the exosome group compared with blank controls (P < 0.05).The miRNA mimic transfection and target gene prediction found that TAM-derived exosomes targeted the miR-146b-5p/TRAF6/NF-κB/MMP2 pathway to suppress endothelial cell migration; this result was supported by PCR and Western blotting analyses. The expression of exosomal miR-146b-5p isolated from serum in the EOC group was significantly increased compared to healthy individuals. Finally, TAM-derived exosomes and EOC SKOV3-derived exosomes in combination stimulated HUVEC cells and overcame the inhibition of endothelial cell migration caused by TAM-derived exosomes. Two lncRNAs that were carried by SKOV3-derived exosomes were identified as NF-κB pathway-associated genes by Western blotting.

conclusionTAM-derived exosomes can inhibit the migration of endothelial cells by targeting the miR-146b-5p/TRAF6/NF-kB/MMP2 pathway. However, EOC-derived exosomes can transfer lncRNAs to remotely reverse this effect of TAMs on endothelial cells.

Indexed as

Endothelial cellEpithelial ovarian cancerExosomeMigrationTumor associated macrophage

Identifiers

PMID28592924
PMCPMC5461704
OpenAlexW2623858099

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.