ArticleScientific reports2017
Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 71 citations in OpenAlex.
- Biophysical and functional characterization of the Escherichia coli Dr fimbrial envelope reveals its importance for pathogenesis and lithogenesis.PLoS pathogens · 2026Article
- Neutrophil Secretory Proteins Inhibit Calcium Oxalate Crystallisation and Crystal Growth, but Promote Crystal Aggregation.Immunology · 2026Article
- Unveiling potential natural promoters of calcium oxalate kidney stones in the urine via anion-exchange chromatography, crystal assays, and proteomics.Molecular and cellular biochemistry · 2026Article
- Sex Differences in Admission Urine Culture Positivity, Pathogen Distribution, and Clinical Characteristics Among Patients with Calcium Oxalate Stones.Pathogens (Basel, Switzerland) · 2026Article
- Urinary proteins from stone formers promote calcium oxalate crystallization, growth and aggregation via oxidative modifications.Journal of advanced research · 2026Article
- Systematic evaluation and comparison of theCurrent research in food science · 2026Article
- Role of UropathogenicPathogens (Basel, Switzerland) · 2025Review
- Effect of long-term postoperative OM-89 administration on bacteriuria from suspected infectious stones.Investigative and clinical urology · 2025Article
- Review
- Dysbiosis of gut and urinary microbiota in urolithiasis patients and post-surgical cases.Frontiers in cellular and infection microbiology · 2025Article
- Anion exchange chromatographic fractionation of urinary proteins followed by tandem mass spectrometry identifies potential natural inhibitors of calcium oxalate stone.Computational and structural biotechnology journal · 2025Article
- The direct inhibitory effects of Lactobacillus acidophilus, a commensal urinary bacterium, on calcium oxalate stone development.Microbiome · 2024Article
- Resveratrol inhibits calcium oxalate crystal growth, reduces adhesion to renal cells and induces crystal internalization into the cells, but promotes crystal aggregation.Current research in food science · 2024Article
- Article
- Kidney Stone Prevention.Advances in nutrition (Bethesda, Md.) · 2023Review
- Sleep and circadian rhythm disturbance in kidney stone disease: a narrative review.Frontiers in endocrinology · 2023Review
- Calcineurin B inhibits calcium oxalate crystallization, growth and aggregation via its high calcium-affinity property.Computational and structural biotechnology journal · 2023Article
- Extraintestinal pathogenicVirulence · 2022Article
- Effects of Escherichia coli andDiagnostics (Basel, Switzerland) · 2022Article
- 16S rRNA gene sequencing reveals altered composition of gut microbiota in postoperative individuals with renal stones.Letters in applied microbiology · 2022Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100 stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation.
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