Evidence map›Paper›PMID 28592550›Full record

ArticleJournal of clinical microbiology2017

Validation and Implementation of Clinical Laboratory Improvements Act-Compliant Whole-Genome Sequencing in the Public Health Microbiology Laboratory.

Varvara K Kozyreva, Chau-Linda Truong, Alexander L Greninger, John Crandall, Rituparna Mukhopadhyay, Vishnu Chaturvedi

Open access · hybridAbstract readValidation Study
In one paragraph

Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
8.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed, 93 citations in OpenAlex.

  1. Review
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  12. International journal of environmental research and public health · 2023
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  14. Discovery of a novel sub-lineage of multi-drug resistant Shigella flexneri in Southern California.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2023
    Article
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  18. Comparative genomics analysis ofFrontiers in cellular and infection microbiology · 2023
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Varvara K KozyrevaMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA.
Chau-Linda TruongMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA.
Alexander L GreningerMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA.
John CrandallMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA.
Rituparna MukhopadhyayMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA.
Vishnu ChaturvediMicrobial Diseases Laboratory, California Department of Public Health, Richmond, California, USA Vishnu.Chaturvedi@cdph.ca.gov.
California Department of Public Health · US

Funding

NCEZID CDC HHS U50 CK000410
6 · The paper itself

Abstract

Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the validation of WGS on the Illumina platform for routine use in PHLs according to Clinical Laboratory Improvements Act (CLIA) guidelines for laboratory-developed tests (LDTs). We developed a validation panel comprising 10

Indexed as

Bacterial InfectionsBacteriological TechniquesHumansMolecular Diagnostic TechniquesMolecular EpidemiologyReproducibility of ResultsSensitivity and SpecificityWhole Genome Sequencingbacteriabioinformatics pipelineCLIAlaboratory-developed testperformance specificationspublic healthquality managementvalidationWGSwhole-genome sequencing

Identifiers

PMID28592550
PMCPMC5527429
OpenAlexW2624618183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.