ArticleNucleic acids research2017
New insights into a classic aptamer: binding sites, cooperativity and more sensitive adenosine detection.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 165 citations in OpenAlex.
- Matrix-Activated Aptamer Sensors for Human Serum Analysis.Angewandte Chemie (International ed. in English) · 2026Article
- Self-Calibrating Ratiometric DNA Aptamer Probe for Quantitative ATP Imaging in Living Cells.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Progress in Aptamer Research and Future Applications.ChemistryOpen · 2025Review
- Dissociation Constant (KSmall methods · 2025Review
- Cost-effective, user-friendly detection and preconcentration of thrombin on a sustainable paper-based electrochemical platform.Analytical and bioanalytical chemistry · 2025Article
- Generalizable Molecular Switch Designs forAccounts of chemical research · 2025Article
- Genetically Encoded Fluorogenic DNA Aptamers for Imaging Metabolite in Living Cells.Journal of the American Chemical Society · 2025Article
- Mechanism of Dual-Site Recognition in a Classic DNA Aptamer.Journal of chemical information and modeling · 2024Article
- In vitro selection of DNA aptamers against staphylococcal enterotoxin A.Scientific reports · 2024Article
- Based on mutated aptamer-smartphone colorimetric detection of metronidazole in milk.Frontiers in bioengineering and biotechnology · 2024Article
- Functional Nucleic Acid Probes Based on Two-Photon for Biosensing.Biosensors · 2023Review
- DNA Aptamer Beacon Probe (ABP) for Monitoring of Adenosine Triphosphate Level in SW480 Cancer Cells Treated with Glycolysis Inhibitor 2-Deoxyglucose.International journal of molecular sciences · 2023Article
- Article
- Critical Design Factors for Electrochemical Aptasensors Based on Target-Induced Conformational Changes: The Case of Small-Molecule Targets.Biosensors · 2022Review
- Overcoming Major Barriers to Developing Successful Sensors for Practical Applications Using Functional Nucleic Acids.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- Unraveling the binding mode of a methamphetamine aptamer: A spectroscopic and calorimetric study.Biophysical journal · 2022Article
- Article
- Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex.Mikrochimica acta · 2022Article
- Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DNA aptamer for adenosine (also for AMP and ATP) is a highly conserved sequence that has recurred in a few selections. It it a widely used model aptamer for biosensor development, and its nuclear magnetic resonance structure shows that each aptamer binds two AMP molecules. In this work, each binding site was individually removed by rational sequence design, while the remaining site still retained a similar binding affinity and specificity as confirmed by isothermal titration calorimetry. The thermodynamic parameters of binding are presented, and its biochemical implications are discussed. The number of binding sites can also be increased, and up to four sites are introduced in a single DNA sequence. Finally, the different sequences are made into fluorescent biosensors based on the structure-switching signaling aptamer design. The one-site aptamer has 3.8-fold higher sensitivity at lower adenosine concentration with a limit of detection of 9.1 μM adenosine, but weaker fluorescence signal at higher adenosine concentrations, consistent with a moderate cooperativity in the original aptamer. This work has offered insights into a classic aptamer for the relationship between the number of binding sites and sensitivity, and a shorter aptamer for improved biosensor design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.