ArticleFEMS microbiology reviews2017
Novel approaches in function-driven single-cell genomics.
Article in FEMS microbiology reviews, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- A genomic view of Earth's biomes.Nature reviews. Genetics · 2026Review
- Fluorometric Analysis of Carrier-Protein-Dependent Biosynthesis through a Conformationally Sensitive Solvatochromic Pantetheinamide Probe.ACS chemical biology · 2024Article
- scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling.mSystems · 2023Article
- Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium.Microbiome · 2023Article
- Functional characterization of prokaryotic dark matter: the road so far and what lies ahead.Current research in microbial sciences · 2022Review
- Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics.ACS chemical biology · 2021Article
- Microbial Community Response to Polysaccharide Amendment in Anoxic Hydrothermal Sediments of the Guaymas Basin.Frontiers in microbiology · 2021Article
- The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment.Water research X · 2020Review
- Genome-Centric Metagenomic Insights into the Impact of Alkaline/Acid and Thermal Sludge Pretreatment on the Microbiome in Digestion Sludge.Applied and environmental microbiology · 2020Article
- Microbial single-cell omics: the crux of the matter.Applied microbiology and biotechnology · 2020Review
- Single-Cell Genomics and the Oral Microbiome.Journal of dental research · 2020Review
- Next-generation physiology approaches to study microbiome function at single cell level.Nature reviews. Microbiology · 2020Review
- Complementary Metagenomic Approaches Improve Reconstruction of Microbial Diversity in a Forest Soil.mSystems · 2020Article
- Function-driven single-cell genomics uncovers cellulose-degrading bacteria from the rare biosphere.The ISME journal · 2020Article
- Targeted Cell Sorting Combined With Single Cell Genomics Captures Low Abundant Microbial Dark Matter With Higher Sensitivity Than Metagenomics.Frontiers in microbiology · 2020Article
- Genome and pan-genome analysis to classify emerging bacteria.Biology direct · 2019Review
- The trajectory of microbial single-cell sequencing.Nature methods · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deeper sequencing and improved bioinformatics in conjunction with single-cell and metagenomic approaches continue to illuminate undercharacterized environmental microbial communities. This has propelled the 'who is there, and what might they be doing' paradigm to the uncultivated and has already radically changed the topology of the tree of life and provided key insights into the microbial contribution to biogeochemistry. While characterization of 'who' based on marker genes can describe a large fraction of the community, answering 'what are they doing' remains the elusive pinnacle for microbiology. Function-driven single-cell genomics provides a solution by using a function-based screen to subsample complex microbial communities in a targeted manner for the isolation and genome sequencing of single cells. This enables single-cell sequencing to be focused on cells with specific phenotypic or metabolic characteristics of interest. Recovered genomes are conclusively implicated for both encoding and exhibiting the feature of interest, improving downstream annotation and revealing activity levels within that environment. This emerging approach has already improved our understanding of microbial community functioning and facilitated the experimental analysis of uncharacterized gene product space. Here we provide a comprehensive review of strategies that have been applied for function-driven single-cell genomics and the future directions we envision.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.