ArticleBiophysical journal2017
Investigation of Nanoscopic Phase Separations in Lipid Membranes Using Inverse FCS.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Reversible Kinetic Trapping of FUS Biomolecular Condensates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Liquid-Liquid Phase Separation: Unraveling the Enigma of Biomolecular Condensates in Microbial Cells.Frontiers in microbiology · 2021Review
- Phase separation at the nanoscale quantified by dcFCCS.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Dynamic pattern generation in cell membranes: Current insights into membrane organization.Biochimica et biophysica acta. Biomembranes · 2018Review
- Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS".Biophysical journal · 2017Article
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5 authors.
Funding
Abstract
Measurement of the sizes of nanoscopic particles is a difficult challenge, especially in two-dimensional systems such as cell membranes. We have extended inverse fluorescence correlation spectroscopy (iFCS) to endow it with unique advantages for measuring particle size from the nano- to the microscale. We have augmented iFCS with an analysis of moments of fluorescence fluctuations and used it to measure stages of phase separation in model lipid bilayer membranes. We observed two different pathways for the growth of phase domains. In one, nanoscopic gel domains appeared first and then gradually grew to micrometer size. In the other, the domains reached micrometer size quickly, and their number gradually increased. These measurements demonstrate the value of iFCS measurements through their ability, to our knowledge, to provide new information about the mechanism of lipid phase separation and potentially about the physical basis of naturally occurring nanodomains such as lipid rafts.
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