ArticleeLife2017
Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.
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Who cites it
79 citing papers in PubMed, 1 synthesis or guideline pooled it, 128 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Vps1 and select autophagic machinery are required for coenzyme Q uptake and trafficking to mitochondria.The Journal of biological chemistry · 2026Article
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- Mechanisms of Quality Control in GPI-Anchored Proteins: The Central Role of the Lipid Anchor.International journal of molecular sciences · 2026Review
- The Lack of a COPII Cargo Receptor Erv14 Impacts Physiological Functions of the Vacuole in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2026Article
- Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane.The Journal of biological chemistry · 2026Article
- Coupling between sterol and sphingolipid structure in ordered membrane domains.bioRxiv : the preprint server for biology · 2026Article
- Microautophagy: current understanding of its molecular mechanisms and functions.Autophagy reports · 2026Review
- Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1.FEBS letters · 2026Article
- Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane.bioRxiv : the preprint server for biology · 2025Article
- Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation.Science advances · 2025Article
- Review
- Comparative Proteomics of Two Flor Yeasts in Sparkling Wine Fermentation: First Approach.Foods (Basel, Switzerland) · 2025Article
- Definition of phosphatidylinositol 4,5-bisphosphate distribution by freeze-fracture replica labeling.The Journal of cell biology · 2025Article
- Structural and biochemical analysis of ligand binding in yeast Niemann-Pick type C1-related protein.Life science alliance · 2025Article
- Sterol-lipids enable large-scale, liquid-liquid phase separation in bilayer membranes of only two components.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- General autophagy-dependent and -independent lipophagic processes collaborate to regulate the overall level of lipophagy in yeast.Autophagy · 2024Article
- Hsc70 promotes anti-tumor immunity by targeting PD-L1 for lysosomal degradation.Nature communications · 2024Article
- Stress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve.Molecular biology of the cell · 2024Article
- Automated quantification of vacuole fusion and lipophagy inAutophagy · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Niemann-Pick type C is a storage disease caused by dysfunction of NPC proteins, which transport cholesterol from the lumen of lysosomes to the limiting membrane of that compartment. Using freeze fracture electron microscopy, we show here that the yeast NPC orthologs, Ncr1p and Npc2p, are essential for formation and expansion of raft-like domains in the vacuolar (lysosome) membrane, both in stationary phase and in acute nitrogen starvation. Moreover, the expanded raft-like domains engulf lipid droplets by a microautophagic mechanism. We also found that the multivesicular body pathway plays a crucial role in microautophagy in acute nitrogen starvation by delivering sterol to the vacuole. These data show that NPC proteins promote microautophagy in stationary phase and under nitrogen starvation conditions, likely by increasing sterol in the limiting membrane of the vacuole.
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