Evidence map›Paper›PMID 28589989›Full record

ArticleThe Analyst2017

Monitoring focal adhesion kinase phosphorylation dynamics in live cells.

Nur P Damayanti, Kevin Buno, Nagarajan Narayanan, Sherry L Voytik Harbin, Meng Deng, Joseph M K Irudayaraj

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Analyst, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nur P DamayantiBindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA. josephi@purdue.edu and Indiana University School of Medicine, 980 West Walnut St. R3 C312, Indianapolis, Indiana 46202, USA.
Kevin BunoWeldon School of Biomedical Engineering, Collage of Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Nagarajan NarayananBindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA. josephi@purdue.edu.
Sherry L Voytik HarbinWeldon School of Biomedical Engineering, Collage of Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Meng DengBindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA. josephi@purdue.edu.
Joseph M K IrudayarajBindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, USA. josephi@purdue.edu.ORCID http://orcid.org/0000-0002-0630-1520

Funding

Label-free, real-time detection of kinase activity in vitro and in single cells uR21CA157395 · NCI · PURDUE UNIVERSITY · PI IRUDAYARAJ, JOSEPH MK · 2011 to 2013
$610k
NCI NIH HHS R21 CA157395
6 · The paper itself

Abstract

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

Indexed as

Biosensing TechniquesFluorescenceCells, CulturedFocal Adhesion Protein-Tyrosine KinasesHumansMicroscopy, ConfocalPhosphorylationSingle-Cell AnalysisTyrosineFocal Adhesion Protein-Tyrosine KinasesTyrosine

Identifiers

PMID28589989
PMCPMC5531600

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.