ArticleScientific reports2017
Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Arrhythmogenic Risk in iPSC-Derived Cardiomyocytes: Current Limitations and Therapeutic Perspectives.Medicina (Kaunas, Lithuania) · 2025Review
- Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments.Advanced healthcare materials · 2025Article
- The paradoxical role of stem cells in osteosarcoma: from pathogenesis to therapeutic breakthroughs.Frontiers in oncology · 2025Review
- Atelocollagen supports three-dimensional culture of human induced pluripotent stem cells.Molecular therapy. Methods & clinical development · 2024Article
- Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.BMC biotechnology · 2024Article
- Hydrogel-Sheathed hiPSC-Derived Heart Microtissue Enables Anchor-Free Contractile Force Measurement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Fabrication and Cell Culture Applications of Core-Shell Hydrogel Fibers Composed of Chitosan/DNA Interfacial Polyelectrolyte Complexation and Calcium Alginate: Straight and Beaded Core Variations.Advanced healthcare materials · 2023Article
- Engineered T cells from induced pluripotent stem cells: from research towards clinical implementation.Frontiers in immunology · 2023Review
- Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering.Chemical reviews · 2022Review
- In vitro proliferation and long-term preservation of functional primary rat hepatocytes in cell fibers.Scientific reports · 2022Article
- Glomerulus-on-a-Chip: Current Insights and Future Potential Towards Recapitulating Selectively Permeable Filtration Systems.International journal of nephrology and renovascular disease · 2022Review
- Microfiber-shaped building-block tissues with endothelial networks for constructing macroscopic tissue assembly.APL bioengineering · 2019Article
- Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers.Micromachines · 2019Article
- Temporal Observation of Adipocyte Microfiber Using Anchoring Device.Micromachines · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. Several suspension culture systems for human pluripotent stem cell expansion exist; however, it is difficult to control the thickness of cell aggregations in these systems, leading to increased cell death likely caused by limited diffusion of gases and nutrients into the aggregations. Here, we describe a scalable culture system using the cell fiber technology for the expansion of human induced pluripotent stem (iPS) cells. The cells were encapsulated and cultured within the core region of core-shell hydrogel microfibers, resulting in the formation of rod-shaped or fiber-shaped cell aggregations with sustained thickness and high viability. By encapsulating the cells with type I collagen, we demonstrated a long-term culture of the cells by serial passaging at a high expansion rate (14-fold in four days) while retaining its pluripotency. Therefore, our culture system could be used for large-scale expansion of human pluripotent stem cells for use in regenerative medicine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.