Evidence map›Paper›PMID 28587849›Full record

ArticleGene2017

MicroRNA profiling in kidney disease: Plasma versus plasma-derived exosomes.

Jeffrey X Xie, Xiaoming Fan, Christopher A Drummond, Reetam Majumder, Yanmei Xie, Tian Chen, Lijun Liu, Steven T Haller, Pamela S Brewster, Lance D Dworkin and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gene, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed, 2 pooled it
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 2 syntheses or guidelines pooled it, 59 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Extracellular vesicles as mediators and markers of acute organ injury: current concepts.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022
    Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Jeffrey X XieUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Xiaoming FanUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Christopher A DrummondUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Reetam MajumderUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Yanmei XieUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Tian ChenUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Lijun LiuUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Steven T HallerUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Pamela S BrewsterUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Lance D DworkinUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Christopher J CooperUniversity of Toledo College of Medicine, Toledo, OH 43614, USA.
Jiang TianUniversity of Toledo College of Medicine, Toledo, OH 43614, USA. Electronic address: Jiang.Tian@utoledo.edu.
University of Toledo · US

Funding

Na/K-ATPase Reduction in Renal Disease-Related Cardiac DysfunctionR01HL105649 · NHLBI · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI TIAN, JIANG · 2012 to 2016
$1.8M
Evaluating the role of Exogenous administration of micro RNA 29b in preventing Chronic Kidney Disease induced cardiac FibrosisF32DK104615 · NIDDK · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI DRUMMOND, CHRISTOPHER ANSON · 2014 to 2016
$134k
NHLBI NIH HHS R01 HL105649NIDDK NIH HHS F32 DK104615
6 · The paper itself

Abstract

Liquid biopsies have advanced rapidly in recent years for use in diagnostic and prognostic applications. One important aspect of this advancement is the growth in our understanding of microRNA (miRNA) biology. The measurement of miRNAs packaged within exosomes, which are constantly released into the blood stream, may reflect pathological changes within the body. The current study performed miRNA profiling using plasma and plasma-derived exosome samples from two animal models of kidney disease, the 5/6th partial nephrectomy (PNx) and two-kidney-one-clip (2K1C) models. The RT-qPCR-based profiling results revealed that the overall miRNA expression level was much higher in plasma than in plasma-derived exosomes. With 200μl of either plasma or exosomes derived from the same volume of plasma, 629 out of 665 total miRNAs analyzed were detectable in plasma samples from sham-operated rats, while only 403 were detectable in exosomes with a cutoff value set at 35cycles. Moreover, the average miRNA expression level in plasma was about 16-fold higher than that in exosomes. We also found a select subset of miRNAs that were enriched within exosomes. The number of detectable miRNAs from plasma-derived exosomes was increased in rats subjected to PNx or 2K1C surgery compared to sham-operated animals. Importantly, we found that the changes of individual miRNAs measured in plasma had very poor concordance with that measured in plasma-derived exosomes in both animal models, suggesting that miRNAs in plasma and plasma-derived exosomes are differentially regulated in these disease conditions. Interestingly, PNx and 2K1C surgeries induced similar changes in miRNA expression, implying that common pathways were activated in these two disease models. Pathway analyses using DIANA-miRPath v3.0 showed that significantly changed exosomal miRNAs were associated with extracellular matrix (ECM) receptor interaction and mucin type-O-glycan synthesis pathways, which are related with tissue fibrosis and kidney injury, respectively. In conclusion, our results demonstrated that due to the differential changes in miRNAs, the measurement of exosomal miRNAs cannot be replaced by the measurement of miRNAs in plasma, or vice versa. We also showed that a set of miRNAs related with kidney injury and organ fibrosis were dysregulated in plasma-derived exosomes from animal models of kidney disease.

Indexed as

AnimalsDisease Models, AnimalExosomesKidney DiseasesMaleMicroRNAsNephrectomyRatsRats, Sprague-DawleyMicroRNAsBiomarkerExosomeMicroRNA

Identifiers

PMID28587849
PMCPMC5534180
OpenAlexW2620972733

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.