Evidence map›Paper›PMID 28587652›Full record

ArticleSkeletal muscle2017

Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F.

Angela K Peter, Gaynor Miller, Joana Capote, Marino DiFranco, Alhondra Solares-Pérez, Emily L Wang, Jim Heighway, Ramón M Coral-Vázquez, Julio Vergara, Rachelle H Crosbie-Watson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Skeletal muscle, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

Angela K PeterDepartment of Integrative Biology and Physiology, University of California, Los Angeles, 610 Charles E. Young Drive East, Terasaki Life Sciences Building, Los Angeles, CA, 90095, USA.
Gaynor MillerDepartment of Integrative Biology and Physiology, University of California, Los Angeles, 610 Charles E. Young Drive East, Terasaki Life Sciences Building, Los Angeles, CA, 90095, USA.
Joana CapoteDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 610 Charles E. Young Drive East, Terasaki Life Sciences Building, Los Angeles, CA, 90095, USA.
Marino DiFrancoCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Alhondra Solares-PérezSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
Emily L WangDepartment of Integrative Biology and Physiology, University of California, Los Angeles, 610 Charles E. Young Drive East, Terasaki Life Sciences Building, Los Angeles, CA, 90095, USA.
Jim HeighwayCancer Communications and Consultancy Ltd, Knutsford, Cheshire, UK.
Ramón M Coral-VázquezSección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
Julio VergaraCenter for Duchenne Muscular Dystrophy, University of California, Los Angeles, Los Angeles, CA, USA.
Rachelle H Crosbie-WatsonDepartment of Integrative Biology and Physiology, University of California, Los Angeles, 610 Charles E. Young Drive East, Terasaki Life Sciences Building, Los Angeles, CA, 90095, USA. rcrosbie@physci.ucla.edu.
University of California, Los Angeles · USInstituto Politécnico Nacional · MXTerasaki Foundation · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Structure-Function Analysis of SarcospanR01AR048179 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CROSBIE, RACHELLE HOPE · 2001 to 2023
$6.9M
UCLA Muscular Dystrophy Core CenterP30AR057230 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SPENCER, MELISSA JAN · 2009 to 2018
$6.2M
MODULATION OF SARCOPLASMIC RETICULUM CALCIUM RELEASER01AR041802 · NIAMS · BAYLOR COLLEGE OF MEDICINE · PI HAMILTON, SUSAN L, INGALLS, CHRISTOPHER · 1993 to 2014
$5.6M
Muscle Cell Biology, Pathophysiology, and TherapeuticsT32AR065972 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Rachelle Hope Crosbie, THOMAS A. RANDO · 2016 to 2026
$3.9M
Isoform-specific roles of the Na,K-ATPase in skeletal muscleR01AR063710 · NIAMS · UNIVERSITY OF CINCINNATI · PI HEINY, JUDITH A, LINGREL, JERRY B · 2013 to 2017
$2.2M
Training in Molecular, Cellular &Integrative PhysiologyT32GM065823 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TIDBALL, JAMES G · 2003 to 2017
$2.1M
Optogenetic control of skeletal muscle excitabilityR21AR067422 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DI FRANCO, MARINO, VERGARA, JULIO L · 2014 to 2015
$373k
Howard Hughes Medical InstituteNCATS NIH HHS UL1 TR000124NIAMS NIH HHS P30 AR057230NIAMS NIH HHS R01 AR041802NIAMS NIH HHS R01 AR048179NIAMS NIH HHS R01 AR063710NIAMS NIH HHS R21 AR067422NIAMS NIH HHS T32 AR065972NIGMS NIH HHS T32 GM065823
6 · The paper itself

Abstract

backgroundSarcospan (SSPN) is a transmembrane protein that interacts with the sarcoglycans (SGs) to form a tight subcomplex within the dystrophin-glycoprotein complex that spans the sarcolemma and interacts with laminin in the extracellular matrix. Overexpression of SSPN ameliorates Duchenne muscular dystrophy in murine models.

methodsStandard cloning approaches were used to identify nanospan, and nanospan-specific polyclonal antibodies were generated and validated. Biochemical isolation of skeletal muscle membranes and two-photon laser scanning microscopy were used to analyze nanospan localization in muscle from multiple murine models. Duchenne muscular dystrophy biopsies were analyzed by immunoblot analysis of protein lysates as well as indirect immunofluorescence analysis of muscle cryosections.

resultsNanospan is an alternatively spliced isoform of sarcospan. While SSPN has four transmembrane domains and is a core component of the sarcolemmal dystrophin-glycoprotein complex, nanospan is a type II transmembrane protein that does not associate with the dystrophin-glycoprotein complex. We demonstrate that nanospan is enriched in the sarcoplasmic reticulum (SR) fractions and is not present in the T-tubules. SR fractions contain membranes from three distinct structural regions: a region flanking the T-tubules (triadic SR), a SR region across the Z-line (ZSR), and a longitudinal SR region across the M-line (LSR). Analysis of isolated murine muscles reveals that nanospan is mostly associated with the ZSR and triadic SR, and only minimally with the LSR. Furthermore, nanospan is absent from the SR of δ-SG-null (Sgcd

conclusionsAlternative splicing of proteins from the SG-SSPN complex produces δ-SG3, microspan, and nanospan that localize to the ZSR and the triadic SR, where they may play a role in regulating resting calcium levels as supported by previous studies (Estrada et al., Biochem Biophys Res Commun 340:865-71, 2006). Thus, alternative splicing of SSPN mRNA generates three protein isoforms (SSPN, microspan, and nanospan) that differ in the number of transmembrane domains affecting subcellular membrane association into distinct protein complexes.

Indexed as

Alternative SplicingAnimalsCarrier ProteinsHumansMembrane ProteinsMiceMice, Inbred C57BLNeoplasm ProteinsProtein IsoformsProtein TransportSarcoglycanopathiesSarcoglycansSarcoplasmic ReticulumCarrier ProteinsMembrane ProteinsNeoplasm ProteinsProtein IsoformsSarcoglycansSspn protein, mouseDuchenne muscular dystrophyDystrophinLimb girdle muscular dystrophyMicrospanNanospanSarcolemmaSarcoplasmic reticulumSarcospanTransverse tubule

Identifiers

PMID28587652
PMCPMC5461684
OpenAlexW2622554959

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.