ArticleBMC plant biology2017
Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Transcriptomic Signature of Lipid Production in Australian Aurantiochytrium sp. TC20.Marine biotechnology (New York, N.Y.) · 2025Article
- Lipid Droplets in EndosymbioticPlants (Basel, Switzerland) · 2024Review
- Bioinformatics-Based Screening Approach for the Identification and Characterization of Lipolytic Enzymes from the Marine DiatomMarine drugs · 2023Article
- Structural and Functional Annotation of Napin-Like Protein from Momordica charantia to Explore its Medicinal Importance.Biochemical genetics · 2022Article
- Plastidic membrane lipids are oxidized by a lipoxygenase inFrontiers in plant science · 2022Article
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- Development of New Antiproliferative Compound against Human Tumor Cells from the Marine MicroalgaeInternational journal of molecular sciences · 2020Article
- Identification of Low-Abundance Lipid Droplet Proteins in Seeds and Seedlings.Plant physiology · 2020Article
- The MicroalgaPlant physiology · 2020Article
- Lipidome Remodeling and Autophagic Respose in the Arachidonic-Acid-Rich MicroalgaFrontiers in plant science · 2020Article
- Degradation of Lipid Droplets in Plants and Algae-Right Time, Many Paths, One Goal.Frontiers in plant science · 2020Review
- The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.Results and problems in cell differentiation · 2020Article
- Proteomics Analysis of Lipid Droplets from the Oleaginous Alga Chromochloris zofingiensis Reveals Novel Proteins for Lipid Metabolism.Genomics, proteomics & bioinformatics · 2019Article
- Marine Natural Products from Microalgae: An -Omics Overview.Marine drugs · 2019Review
- The green microalga Lobosphaera incisa harbours an arachidonate 15S-lipoxygenase.Plant biology (Stuttgart, Germany) · 2019Article
- The type 2 acyl-CoA:diacylglycerol acyltransferase family of the oleaginous microalga Lobosphaera incisa.BMC plant biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLobosphaera incisa (L. incisa) is an oleaginous microalga that stores triacylglycerol (TAG) rich in arachidonic acid in lipid bodies (LBs). This organelle is gaining attention in algal research, since evidence is accumulating that proteins attached to its surface fulfill important functions in TAG storage and metabolism.
resultsHere, the composition of the LB proteome in L incisa was investigated by comparing different cell fractions in a semiquantitative proteomics approach. After applying stringent filters to the proteomics data in order to remove contaminating proteins from the list of possible LB proteins (LBPs), heterologous expression of candidate proteins in tobacco pollen tubes, allowed us to confirm 3 true LBPs: A member of the algal Major Lipid Droplet Protein family, a small protein of unknown function and a putative lipase. In addition, a TAG lipase that belongs to the SUGAR DEPENDENT 1 family of TAG lipases known from oilseed plants was identified. Its activity was verified by functional complementation of an Arabidopsis thaliana mutant lacking the major seed TAG lipases.
conclusionsHere we describe 3 LBPs as well as a TAG lipase from the oleaginous microalga L. incisa and discuss their possible involvement in LB metabolism. This study highlights the importance of filtering LB proteome datasets and verifying the subcellular localization one by one, so that contaminating proteins can be recognized as such. Our dataset can serve as a valuable resource in the identification of additional LBPs, shedding more light on the intriguing roles of LBs in microalgae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.