ArticleMicrobial cell factories2017
Construction of energy-conserving sucrose utilization pathways for improving poly-γ-glutamic acid production in Bacillus amyloliquefaciens.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 42 citations in OpenAlex.
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- Synthesis of Poly-γ-Glutamic Acid and Its Application in Biomedical Materials.Materials (Basel, Switzerland) · 2023Review
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- Recent Advances in Microbial Synthesis of Poly-γ-Glutamic Acid: A Review.Foods (Basel, Switzerland) · 2022Review
- Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette ofSynthetic and systems biotechnology · 2022Article
- Enhanced sucrose fermentation by introduction of heterologous sucrose transporter and invertase intoRoyal Society open science · 2021Article
- A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13.Communications biology · 2021Article
- High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10.Microbial cell factories · 2020Article
- Construction and analysis of an artificial consortium based on the fast-growing cyanobacteriumBiotechnology for biofuels · 2020Article
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10 authors at 4 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundSucrose is an naturally abundant and easily fermentable feedstock for various biochemical production processes. By now, several sucrose utilization pathways have been identified and characterized. Among them, the pathway consists of sucrose permease and sucrose phosphorylase is an energy-conserving sucrose utilization pathway because it consumes less ATP when comparing to other known pathways. Bacillus amyloliquefaciens NK-1 strain can use sucrose as the feedstock to produce poly-γ-glutamic acid (γ-PGA), a highly valuable biopolymer. The native sucrose utilization pathway in NK-1 strain consists of phosphoenolpyruvate-dependent phosphotransferase system and sucrose-6-P hydrolase and consumes more ATP than the energy-conserving sucrose utilization pathway.
resultsIn this study, the native sucrose utilization pathway in NK-1 was firstly deleted and generated the B. amyloliquefaciens 3Δ strain. Then four combination of heterologous energy-conserving sucrose utilization pathways were constructed and introduced into the 3Δ strain. Results demonstrated that the combination of cscB (encodes sucrose permease) from Escherichia coli and sucP (encodes sucrose phosphorylase) from Bifidobacterium adolescentis showed the highest sucrose metabolic efficiency. The corresponding mutant consumed 49.4% more sucrose and produced 38.5% more γ-PGA than the NK-1 strain under the same fermentation conditions.
conclusionsTo our best knowledge, this is the first report concerning the enhancement of the target product production by introducing the heterologous energy-conserving sucrose utilization pathways. Such a strategy can be easily extended to other microorganism hosts for reinforced biochemical production using sucrose as substrate.
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