ArticleInternational journal of molecular sciences2017
Dynamic In Vivo Profiling of DNA Damage and Repair after Radiotherapy Using Canine Patients as a Model.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Defining the parameters of comet assay for reliably detecting DNA damage as a marker of X-ray based irradiation in insects treated with non-lethal doses for export fruit sanitation.Pest management science · 2025Article
- Involvement of TGF-β, mTOR, and inflammatory mediators in aging alterations during myxomatous mitral valve disease in a canine model.GeroScience · 2025Review
- Why Concurrent CDDP and Radiotherapy Has Synergistic Antitumor Effects: A Review of In Vitro Experimental and Clinical-Based Studies.International journal of molecular sciences · 2021Review
- Target-Based Radiosensitization Strategies: Concepts and Companion Animal Model Outlook.Frontiers in oncology · 2021Review
- Radiotherapy-induced bone deterioration is exacerbated in diabetic rats treated with streptozotocin.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2021Article
- DNA Strand Break Properties of Protoporphyrin IX by X-Ray Irradiation against Melanoma.International journal of molecular sciences · 2020Article
- Holistic View on Cell Survival and DNA Damage: How Model-Based Data Analysis Supports Exploration of Dynamics in Biological Systems.Computational and mathematical methods in medicine · 2020Article
- Emerging Translational Opportunities in Comparative Oncology With Companion Canine Cancers: Radiation Oncology.Frontiers in oncology · 2019Review
- Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro.PloS one · 2019Article
- DNA Injury and Repair Systems.International journal of molecular sciences · 2018Article
- Advantages of evaluating γH2AX induction in non-clinical drug development.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2018Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time resolved data of DNA damage and repair after radiotherapy elucidates the relation between damage, repair, and cell survival. While well characterized in vitro, little is known about the time-course of DNA damage response in tumors sampled from individual patients. Kinetics of DNA damage after radiotherapy was assessed in eight dogs using repeated in vivo samples of tumor and co-irradiated normal tissue analyzed with comet assay and phosphorylated H2AX (γH2AX) immunohistochemistry. In vivo results were then compared (in silico) with a dynamic mathematical model for DNA damage formation and repair. Maximum %DNA in tail was observed at 15-60 min after irradiation, with a rapid decrease. Time-courses of γH2AX-foci paralleled these findings with a small time delay and were not influenced by covariates. The evolutionary parameter search based on %DNA in tail revealed a good fit of the DNA repair model to in vivo data for pooled sarcoma time-courses, but fits for individual sarcoma time-courses suffer from the heterogeneous nature of the in vivo data. It was possible to follow dynamics of comet tail intensity and γH2AX-foci during a course of radiation using a minimally invasive approach. DNA repair can be quantitatively investigated as time-courses of individual patients by integrating this resulting data into a dynamic mathematical model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.