ArticleJournal of experimental botany2017
Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 36 citations in OpenAlex.
- Nucleoside Diphosphate Kinase Family: Evolutionary Analysis and Protective Role in Mitochondrial ROS Production.Plants (Basel, Switzerland) · 2026Article
- The recruitment of the A-type cyclin TAM to stress granules is crucial for meiotic fidelity under heat.Science advances · 2025Article
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- Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism.The New phytologist · 2024Article
- Calmodulin-Domain Protein Kinase PiCDPK1 Interacts with the 14-3-3-like Protein NtGF14 to Modulate Pollen Tube Growth.Plants (Basel, Switzerland) · 2024Article
- Quantifying Protein Dynamics by Kymograph Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Past accomplishments and future challenges of the multi-omics characterization of leaf growth.Plant physiology · 2022Review
- Global proteome analyses of phosphorylation and succinylation of barley root proteins in response to phosphate starvation and recovery.Frontiers in plant science · 2022Article
- Photoaffinity labelling strategies for mapping the small molecule-protein interactome.Organic & biomolecular chemistry · 2021Review
- Histological and proteome analyses of3 Biotech · 2021Article
- Transcriptomic Analysis for the Identification of Metabolic Pathway Genes Related to Toluene Response inPlants (Basel, Switzerland) · 2021Article
- Global mapping of protein-metabolite interactions in Saccharomyces cerevisiae reveals that Ser-Leu dipeptide regulates phosphoglycerate kinase activity.Communications biology · 2021Article
- Label-Free Proteomic Analysis of Smoke-Drying and Shade-Drying Processes of Postharvest Rhubarb: A Comparative Study.Frontiers in plant science · 2021Article
- Diverse roles of nucleoside diphosphate kinase in genome stability and growth fitness.Current genetics · 2020Review
- Nucleotide Metabolism in Plants.Plant physiology · 2020Article
- Emerging strategies for the identification of protein-metabolite interactions.Journal of experimental botany · 2019Article
- Comparative proteomic study of phytotoxic effects of silver nanoparticles and silver ions on tobacco plants.Environmental science and pollution research international · 2019Article
- PROMIS, global analysis of PROtein-metabolite interactions using size separation inThe Journal of biological chemistry · 2018Article
- 2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes.Journal of visualized experiments : JoVE · 2018Article
- Plant nucleoside diphosphate kinase 1: A housekeeping enzyme with moonlighting activity.Plant signaling & behavior · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, analysis of protein-metabolite and protein-protein interactions simultaneously in plant systems. More specifically, we examined protein and small-molecule partners of the three (of five) nucleoside diphosphate kinases present in the Arabidopsis genome (NDPK1-NDPK3). The bona fide role of NDPKs is the exchange of terminal phosphate groups between nucleoside diphosphates (NDPs) and triphosphates (NTPs). However, other functions have been reported, which probably depend on both the proteins and small molecules specifically interacting with the NDPK. Using our approach we identified 23, 17, and 8 novel protein partners of NDPK1, NDPK2, and NDPK3, respectively, with nucleotide-dependent proteins such as actin and adenosine kinase 2 being enriched. Particularly interesting, however, was the co-elution of glutathione S-transferases (GSTs) and reduced glutathione (GSH) with the affinity-purified NDPK1 complexes. Following up on this finding, we could demonstrate that NDPK1 undergoes glutathionylation, opening a new paradigm of NDPK regulation in plants. The described results extend our knowledge of NDPKs, the key enzymes regulating NDP/NTP homeostasis.
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