ArticleNucleic acids research2017
FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- Lighting Up Endogenous RNA: Fluorescent Aptamer Sensors for Live-Cell Imaging.Chemical & biomedical imaging · 2026Review
- A programmable ribozyme for RNA signal transduction.Nature communications · 2026Article
- Investigating the Effect of RNA Scaffolds on the Multicolor Fluorogenic Aptamer Pepper in Different Bacterial Species.ACS synthetic biology · 2024Article
- An RNA origami robot that traps and releases a fluorescent aptamer.Science advances · 2024Article
- A universal orthogonal imaging platform for living-cell RNA detection using fluorogenic RNA aptamers.Chemical science · 2023Article
- RNA-Responsive gRNAs for Controlling CRISPR Activity: Current Advances, Future Directions, and Potential Applications.The CRISPR journal · 2022Review
- Spinach-based RNA mimicking GFP in plant cells.Functional & integrative genomics · 2022Article
- Computational study on the binding of Mango-II RNA aptamer and fluorogen using the polarizable force field AMOEBA.Frontiers in molecular biosciences · 2022Article
- The International Society of RNA Nanotechnology and Nanomedicine (ISRNN): The Present and Future of the Burgeoning Field.ACS nano · 2021Article
- Review
- Recent Advances in Aptamer Sensors.Sensors (Basel, Switzerland) · 2021Review
- Article
- Quantitative, super-resolution localization of small RNAs with sRNA-PAINT.Nucleic acids research · 2020Article
- Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells.Cell chemical biology · 2020Review
- A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering.Nature communications · 2020Article
- RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs.Current opinion in biotechnology · 2020Review
- Self-powered RNA nanomachine driven by metastable structure.Nucleic acids research · 2019Article
- Article
- RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.Angewandte Chemie (International ed. in English) · 2019Review
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), play a variety of important regulatory roles in many eukaryotes. Their small size has made it challenging to study them directly in live cells. Here we describe an RNA-based fluorescent sensor for small RNA detection both in vitro and in vivo, adaptable for any small RNA. It utilizes an sxRNA switch for detection of miRNA-mRNA interactions combined with a fluorophore-binding sequence 'Spinach', a GFP-like RNA aptamer for which the RNA-fluorophore complex exhibits strong and consistent fluorescence under an excitation wavelength. Two example sensors, FASTmiR171 and FASTmiR122, can rapidly detect and quantify the levels of miR171 and miR122 in vitro. The sensors can determine relative levels of miRNAs in total RNA extracts with sensitivity similar to small RNA sequencing and northern blots. FASTmiR sensors were also used to estimate the copy number range of miRNAs in total RNA extracts. To localize and analyze the spatial distribution of small RNAs in live, single cells, tandem copies of FASTmiR122 were expressed in different cell lines. FASTmiR122 was able to quantitatively detect the differences in miR122 levels in Huh7 and HEK293T cells demonstrating its potential for tracking miRNA expression and localization in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.