ArticleThe Journal of infectious diseases2017
A Sensitive Method for Detecting Zika Virus Antigen in Patients' Whole-Blood Specimens as an Alternative Diagnostic Approach.
Article in The Journal of infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.ACS synthetic biology · 2025Article
- Investigation of the Influence of the Extraction System and Seasonality on the Pharmacological Potential ofMolecules (Basel, Switzerland) · 2025Article
- Zika Virus Replication in Myeloid Cells during Acute Infection Is Vital to Viral Dissemination and Pathogenesis in a Mouse Model.Journal of virology · 2020Article
- Type I interferon shapes the quantity and quality of the anti-Zika virus antibody response.Clinical & translational immunology · 2020Article
- Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells.Nature communications · 2019Article
- An update on the aspects of Zika virus infection on male reproductive system.Journal of assisted reproduction and genetics · 2019Review
- Zika Virus Infection, Basic and Clinical Aspects: A Review Article.Iranian journal of public health · 2019Review
- Transcriptome Profiling Reveals Pro-Inflammatory Cytokines and Matrix Metalloproteinase Activation in Zika Virus Infected Human Umbilical Vein Endothelial Cells.Frontiers in pharmacology · 2019Article
- Novel differential linear B-cell epitopes to identify Zika and dengue virus infections in patients.Clinical & translational immunology · 2019Article
- Multimodal assessments of Zika virus immune pathophysiological responses in marmosets.Scientific reports · 2018Article
- Longitudinal Study of Cellular and Systemic Cytokine Signatures to Define the Dynamics of a Balanced Immune Environment During Disease Manifestation in Zika Virus-Infected Patients.The Journal of infectious diseases · 2018Article
- Detection of Specific ZIKV IgM in Travelers Using a Multiplexed Flavivirus Microsphere Immunoassay.Viruses · 2018Article
- Silent infection of human dendritic cells by African and Asian strains of Zika virus.Scientific reports · 2018Article
- Zika viral infection and neutralizing human antibody response in a BLT humanized mouse model.Virology · 2018Article
- Advances in Diagnosis, Surveillance, and Monitoring of Zika Virus: An Update.Frontiers in microbiology · 2017Review
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Authors and funding
13 authors at 8 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Epidemics caused by the reemergence of Zika virus (ZIKV) warrant the need to develop new diagnostic measures to complement currently used detection methods. In this study, we explored the detection of ZIKV antigen in a defined leukocyte subset from patients' whole-blood specimens. Methods: Whole-blood samples were obtained at the acute and early convalescent phases from ZIKV-infected patients during the Singapore outbreak in August-September 2016. Presence of ZIKV antigen was determined by flow cytometry staining for intracellular ZIKV NS3, using a ZIKV-specific polyclonal antibody. The presence of ZIKV antigen was determined in CD45+CD14+ monocytes. Results: Data showed that ZIKV NS3 antigen could be detected in CD45+CD14+ monocytes. The levels of detection were further categorized into 3 groups: high (positivity among >40% of monocytes), moderate (positivity among 10%-40%), and low (positivity among <10%). While a majority of patients showed a decrease in the amount of ZIKV antigen detected at later time points, some patients displayed higher levels as the disease progressed. Conclusions: Our data highlights an alternative approach in using flow cytometry as a sensitive method for detecting ZIKV antigen in whole blood. Importantly, it further confirms the role of CD14+ monocytes as an important cellular target for ZIKV infection during the viremic phase.
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