Evidence map›Paper›PMID 28585867›Full record

ArticleDrug delivery2017

Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice.

Natsuko Oyama, Yuki Fuchigami, Shintaro Fumoto, Megumu Sato, Masayori Hagimori, Kazunori Shimizu, Shigeru Kawakami

Open access · goldAbstract read
In one paragraph

Article in Drug delivery, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Natsuko Oyamaa Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.
Yuki Fuchigamia Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.
Shintaro Fumotoa Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.ORCID http://orcid.org/0000-0002-2218-4547
Megumu Satoa Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.
Masayori Hagimoria Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.ORCID http://orcid.org/0000-0002-6718-2422
Kazunori Shimizub Graduate School of Engineering , Nagoya University , Nagoya , Japan.
Shigeru Kawakamia Graduate School of Biomedical Sciences , Nagasaki University , Nagasaki , Japan.ORCID http://orcid.org/0000-0001-8765-2463
Nagasaki University · JPNagoya University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have previously developed an efficient and safe transfection method for the kidney in mice: renal suction-mediated transfection. In this study, we verified the detailed characteristics of transgene expression and plasmid DNA (pDNA) in mice to develop therapeutic strategies and application to gene function analysis in the kidney. After naked pDNA was administered intravenously, the right kidney was immediately suctioned by a tissue suction device. We examined the spatial distribution of transgene expression and pDNA in the suctioned kidney using tissue clearing by CUBIC, Clear

Indexed as

AnimalsDNALuciferasesMicePlasmidsTransfectionTransgenesDNALuciferasesfibroblastGene deliverykidneynaked pDNAspatial distribution

Identifiers

PMID28585867
PMCPMC8241128
OpenAlexW2621932521

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.