Evidence map›Paper›PMID 28585084›Full record

ArticleAnalytical and bioanalytical chemistry2017

Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.

Asif Shajahan, Christian Heiss, Mayumi Ishihara, Parastoo Azadi

Abstract read
In one paragraph

Article in Analytical and bioanalytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.

0numbers the graph read from it
0cells of the map it votes in
73citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

73 citing papers in PubMed.

  1. Review
  2. Physical, chemical, and structural properties of human gastric organoid-derived mucus.American journal of physiology. Gastrointestinal and liver physiology · 2026
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  4. Review
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  13. Article
  14. Characterization of Ssc, anJournal of bacteriology · 2024
    Article
  15. Article
  16. Article
  17. Review
  18. Label-Free Liquid Chromatography-Mass Spectrometry Quantitation of RelativeInternational journal of molecular sciences · 2024
    Article
  19. Article
  20. Article

13 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Asif ShajahanComplex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA.
Christian HeissComplex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA.
Mayumi IshiharaComplex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA.
Parastoo AzadiComplex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA. azadi@ccrc.uga.edu.

Funding

Orbitrap Fusion Tribrid Mass SpectrometerS10OD018530 · OD · UNIVERSITY OF GEORGIA · PI AZADI, PARASTOO · 2014 to 2014
$750k
Glycan linkage and sequence plus determination of site of glycosylation by permethylation of glycopeptides and MSn analysis in a one pot experimentR21GM122633 · NIGMS · UNIVERSITY OF GEORGIA · PI AZADI, PARASTOO · 2016 to 2017
$582k
NIGMS NIH HHS R21 GM122633NIH HHS S10 OD018530
6 · The paper itself

Abstract

The structural analysis of glycoproteins is a challenging endeavor and is under steadily increasing demand, but only a very limited number of labs have the expertise required to accomplish this task. This tutorial is aimed at researchers from the fields of molecular biology and biochemistry that have discovered that glycoproteins are important in their biological research and are looking for the tools to elucidate their structure. It provides brief descriptions of the major and most common analytical techniques used in glycomics and glycoproteomics analysis, including explanations of the rationales for individual steps and references to published literature containing the experimental details necessary to carry out the analyses. Glycomics includes the comprehensive study of the structure and function of the glycans expressed in a given cell or organism along with identification of all the genes that encode glycoproteins and glycosyltransferases. Glycoproteomics which is subset of both glycomics and proteomics is the identification and characterization of proteins bearing carbohydrates as posttranslational modification. This tutorial is designed to ease entry into the glycomics and glycoproteomics field for those without prior carbohydrate analysis experience.

Indexed as

Amino Acid SequenceGlycomicsGlycoproteinsHumansProteomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationGlycoproteinsGlycan analysisGlycomicsGlycopeptideGlycoproteomicsGlycosylation site mappingMass spectrometry

Identifiers

PMID28585084
PMCPMC5498624

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.