ArticlePlant cell reports2017
Deletion of a target gene in Indica rice via CRISPR/Cas9.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- TKC-MC: An Effective Strategy for Generating Heritable Heterozygous Mutations in Essential Genes in Rice.Plant biotechnology journal · 2026Article
- Tissue culture optimization and genome editing for yield improvement of an Indian rice landrace Chittimuthyalu.Transgenic research · 2025Article
- Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing.Nature plants · 2025Article
- Application of genome editing techniques to regulate gene expression in crops.BMC plant biology · 2024Review
- Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs.Horticulture research · 2023Article
- Codon usage bias in chloroplast genes implicate adaptive evolution of four ginger species.Frontiers in plant science · 2023Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Modified Gene Editing Systems: Diverse Bioengineering Tools and Crop Improvement.Frontiers in plant science · 2022Review
- Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens.Plant molecular biology · 2021Article
- Control of Plant Viral Diseases by CRISPR/Cas9: Resistance Mechanisms, Strategies and Challenges in Food Crops.Plants (Basel, Switzerland) · 2021Review
- Modification of cereal plant architecture by genome editing to improve yields.Plant cell reports · 2021Review
- CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility inFrontiers in plant science · 2021Article
- Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer.Scientific reports · 2020Article
- An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in Grains.Rice (New York, N.Y.) · 2020Article
- Dual sgRNA-directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system.Microbial biotechnology · 2020Article
- Heterotrimeric G protein are involved in the regulation of multiple agronomic traits and stress tolerance in rice.BMC plant biology · 2020Article
- CRISPR-associated nucleases: the Dawn of a new age of efficient crop improvement.Transgenic research · 2020Review
- Identification of Multiple Grain Shape-Related Loci in Rice Using Bulked Segregant Analysis With High-Throughput Sequencing.Frontiers in plant science · 2020Article
- Efficient and modular CRISPR-Cas9 vector system forPlant direct · 2019Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageUsing CRISPR/Cas9, we successfully deleted large fragments of the yield-related gene DENSE AND ERECT PANICLE1 in Indica rice at relatively high frequency and generated gain-of-function dep1 mutants. CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a rapidly developing technology used to produce gene-specific modifications in both mammalian and plant systems. Most CRISPR-induced modifications in plants reported to date have been small insertions or deletions. Few large target gene deletions have thus far been reported, especially for Indica rice. In this study, we designed multiple CRISPR sgRNAs and successfully deleted DNA fragments in the gene DENSE AND ERECT PANICLE1 (DEP1) in the elite Indica rice line IR58025B. We achieved deletion frequencies of up to 21% for a 430 bp target and 9% for a 10 kb target among T0 events. Constructs with four sgRNAs did not generate higher full-length deletion frequencies than constructs with two sgRNAs. The multiple mutagenesis frequency reached 93% for four targets, and the homozygous mutation frequency reached 21% at the T0 stage. Important yield-related trait characteristics, such as dense and erect panicles and reduced plant height, were observed in dep1 homozygous T0 mutant plants produced by CRISPR/Cas9. Therefore, we successfully obtained deletions in DEP1 in the Indica background using the CRISPR/Cas9 editing tool at relatively high frequency.
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