ArticleJournal of forensic dental sciences
Sex determination efficacy of Papanicolaou and acriflavine Schiff stains in buccal smears.
Article in Journal of forensic dental sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Determination of the Prevalence of Barr Body in Buccal Smear in the East Khasi Hills Population.Cureus · 2026Article
- Oral Pathology in Forensic Investigation.Journal of International Society of Preventive & Community DentistryReview
- Comparative analysis of cheiloscopy, pulpal tissue and fingerprint for gender identification.Journal of oral and maxillofacial pathology : JOMFPArticle
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBarr body is formed from random inactivation and condensation of one of the two female chromosomes in virtually all the somatic cells of female mammals. Buccal smears have been reported to be potential sources of Barr bodies.
aimThis study was done to assess the efficacy of acriflavine (AF) Schiff and Papanicolaou (PAP) stains in sex determination by identifying Barr bodies in buccal smears of both sexes. MATERIALS AND
methodsTwo samples of buccal smears, collected from thirty males and thirty females in the age group of 16-60 years were used to demonstrate Barr bodies using AF Schiff and PAP stains, respectively. Hundred cells were examined for Barr body positive nucleus, and its mean percentage was calculated and statistically analyzed.
resultsIn females, AF Schiff stained positive cells ranged from 16% to 53% and PAP stained positive cells ranged from 9% to 38%. In males, 0-9% AF positive Barr bodies and 0-5% PAP stained Barr bodies were identified.
conclusionSex determination using buccal smear is a simple and reliable method. AF Schiff stain is better both qualitatively and quantitatively when compared to PAP stain, thus aids in more accurate sex determination.
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