ArticleJournal of experimental botany2017
A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis.
Article in Journal of experimental botany, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
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- Molecular Mechanisms of Plant Responses to Salt Stress.Frontiers in plant science · 2022Review
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- Screening of protonstatin-1 (PS-1) analogs for improved inhibitors of plant plasma membrane HFrontiers in plant science · 2022Article
- Defective cytokinin signaling reprograms lipid and flavonoid gene-to-metabolite networks to mitigate high salinity inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phospholipids in Salt Stress Response.Plants (Basel, Switzerland) · 2021Review
- Nanoalgosomes: Introducing extracellular vesicles produced by microalgae.Journal of extracellular vesicles · 2021Article
- An improved protein lipid overlay assay for studying lipid-protein interactions.Plant methods · 2020Article
- Root-zone-specific sensitivity of K+-and Ca2+-permeable channels to H2O2 determines ion homeostasis in salinized diploid and hexaploid Ipomoea trifida.Journal of experimental botany · 2019Article
- Integrated physiologic, proteomic, and metabolomic analyses ofHorticulture research · 2019Article
- Involvement of Phosphatidylserine and Triacylglycerol in the Response of Sweet Potato Leaves to Salt Stress.Frontiers in plant science · 2019Article
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6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma membrane (PM) H+-ATPase is essential for plant growth and development. Various environmental stimuli regulate its activity, a process that involves many protein cofactors. However, whether endogenous small molecules play a role in this regulation remains unknown. Here, we describe a bio-guided isolation method to identify endogenous small molecules that regulate PM H+-ATPase activity. We obtained crude extracts from Arabidopsis seedlings with or without salt treatment and then purified them into fractions based on polarity and molecular mass by repeated column chromatography. By evaluating the effect of each fraction on PM H+-ATPase activity, we found that fractions containing the endogenous, free unsaturated fatty acids oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3) extracted from salt-treated seedlings stimulate PM H+-ATPase activity. These results were further confirmed by the addition of exogenous C18:1, C18:2, or C18:3 in the activity assay. The ssi2 mutant, with reduced levels of C18:1, C18:2, and C18:3, displayed reduced PM H+-ATPase activity. Furthermore, C18:1, C18:2, and C18:3 directly bound to the C-terminus of the PM H+-ATPase AHA2. Collectively, our results demonstrate that the binding of free unsaturated fatty acids to the C-terminus of PM H+-ATPase is required for its activation under salt stress. The bio-guided isolation model described in this study could enable the identification of new endogenous small molecules that modulate essential protein functions, as well as signal transduction, in plants.
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